产肌酐水解酶基因工程菌的构建  

Construction of Gene Engineering Bacterium Expressing Creatinine Hydrolase

作　　者：程新[1] 张彦新[1] 周桂凤[2] 蒋云生[1] 

机构地区：[1]中南大学湘雅二医院肾病研究所,长沙410011 [2]湖南师范大学医学院,长沙400067

出　　处：《生物医学工程研究》2009年第4期299-302,共4页Journal Of Biomedical Engineering Research

基　　金：国家自然科学基金资助项目(30670974)

摘　　要：从恶臭假单胞菌基因组中PCR扩增到肌酐水解酶基因,并构建成含有肌酐水解酶基因的重组质粒PMG36e-C,重组质粒PMG36e-C电转化保加利亚乳酸成功构建了含肌酐水解酶的基因工程乳酸菌。SDS-PAGE测定基因工程菌合成的肌酐水解酶亚基分子量约为28 KD,在体外测定菌酶液活性可达0.19 u/mL,为开发具有分解肌酐的对尿毒症有治疗作用的可食用基因工程菌奠定基础。The Creatinine hydrolase gene from Pseudomonas Putida genome was amplificated and the recombinant plasmid PMG36e-C containing the Creatinine hydrolase gene was constructed.The recombinant plasmid PMG36e-C was electrotransformed to Lactobacillus Bulgaria and then the genetic engineering bacteria containing the Creatinine hydrolase gene was constructed successfully.SDS-PAGE determination of Creatinine hydrolase subunit molecular weight is about 28 KD.In vitro acticity determination of Enzyme synthesized by re...

关 键 词：基因克隆 基因工程菌 肌酐水解酶 乳酸菌 

分 类 号：R318[医药卫生—生物医学工程]