Time-resolved fluoroimmunoassay of zearalenone in cereals with a europium chelate as label  

作　　者：马智鸿 黄飚 张珏 张艺 朱岚 屠蔷 

机构地区：[1]Jiangsu Institute of Nuclear Medicine,Key Laboratory of Nuclear Medicine,Ministry of Health [2]Department of Pharmaceutical and Molecular Biotechnology,School of Biotechnology,Key Laboratory of Industrial Biotechnology,Ministry of Education,Southern Yangtze University

出　　处：《Journal of Rare Earths》2009年第6期1088-1091,共4页稀土学报（英文版）

基　　金：supported by the Innovation Fund for Technology Based Firms (06C26213201075);National High Technology Research and Development Program of China (863 Program) (2008AA102415)

摘　　要：A competitive indirect time-resolved fluoroimmunoassay(TRFIA) was developed for detection of zearalenone(ZEN) in cereals,in which ZEN conjugated to bovine serum albumin(BSA) is used as solid-phase antigen.A competitive indirect TRFIA was conducted by simultaneously incubating ZEN in standard or extracted samples with anti-ZEN monoclonal antibody over ZEN-BSA coated plates,and then determining the bound ZEN monoclonal antibody with goat anti-mouse europium conjugate.Samples were extracted with methanol/water...A competitive indirect time-resolved fluoroimmunoassay(TRFIA) was developed for detection of zearalenone(ZEN) in cereals,in which ZEN conjugated to bovine serum albumin(BSA) is used as solid-phase antigen.A competitive indirect TRFIA was conducted by simultaneously incubating ZEN in standard or extracted samples with anti-ZEN monoclonal antibody over ZEN-BSA coated plates,and then determining the bound ZEN monoclonal antibody with goat anti-mouse europium conjugate.Samples were extracted with methanol/water...

关 键 词：time-resolved fluoroimmunoassay ZEARALENONE EUROPIUM rare earths food safety 

分 类 号：S379.1[农业科学—农产品加工]