柱前衍生化-高效液相色谱法跟踪检测酶法制备L-蛋氨酸反应过程  

Analysis of L-Methionine in the enzymatic synthesis procedure by high performance liquid chromatography

作　　者：吴燕娇[1] 屠春燕[1] 赵文君[1] 李环[1] 唐美华[1] 韦萍[1] 李寿椿[2] 

机构地区：[1]南京工业大学生物与制药工程学院,南京210009 [2]南京中意化工技术开发研究所,南京210037

出　　处：《分析试验室》2010年第8期24-27,共4页Chinese Journal of Analysis Laboratory

基　　金：国家重点基础研究发展计划(2009CB724700)项目资助

摘　　要：以邻硝基苯磺酰氯为柱前衍生试剂,RP-HPLC为分离手段,选用Lichrospher C18柱,15 mmol/L的磷酸盐溶液(pH5.0)和乙腈为流动相,梯度洗脱,检测波长230 nm,建立了柱前衍生-高效液相色谱法测定N-乙酰-DL-蛋氨酸和L-蛋氨酸的方法,所有组分于20min内洗脱结束。N-乙酰-DL-蛋氨酸和L-蛋氨酸在2.75～254μmol/L范围内线性良好,检出限分别为153 nmol/L和55 nmol/L。加标回收率分别为101.1%和99.8%,相对标准偏差为1.4%和0.89%。本方法可用于酶法或固定化酶法制备蛋氨酸反应体系的跟踪分析。In this paper,a liquid chromatographic(RP-HPLC) method was developed for the analysis of N-acetyl-DL-methionine and L-Methionine(L-Met).Under the optimal conditions,a Lichrospher C18 column(5 μm,4.6 mm×250 mm) was used,and the detection wavelength was 230 nm.Flow rate was 1.0 mL/min,the mobile phase was acetonitrile and 15mmol/L sodium dihydrogen phosphate,and the gradient protocol was applied.All compounds were completely eluted in 20 min.The linear ranges were 2.75～254 μmol/L for N-acetyl-DL-methionine an...

关 键 词：L-蛋氨酸 邻硝基苯磺酰氯 RP-HPLC 衍生化 

分 类 号：TQ922.9[轻工技术与工程—发酵工程]