持续静压力对大鼠成纤维样滑膜细胞PRG4表达的影响  

The effects of hydrostatic pressure on PRG4 production in rat synovial fibroblasts

作　　者：冯剑颖[1] 谷志远[2] 林新平[3] 吴梦婕[1] 

机构地区：[1]浙江大学医学院附属口腔医院正畸科,主治医师浙江310006 [2]浙江大学医学院附属口腔医院口腔颌面外科,副院长教授浙江3100006 [3]浙江大学医学院附属口腔医院口腔正畸科,主任主任医师浙江310006

出　　处：《口腔颌面修复学杂志》2010年第2期75-78,共4页Chinese Journal of Prosthodontics

基　　金：国家自然科学基金资助项目(项目编号:30872901)

摘　　要：目的:探讨大鼠滑膜细胞在持续静压力作用下PRG4蛋白和mRNA的表达变化。方法:原代培养大鼠颞颌关节滑膜细胞,对传代细胞施加持续静水压力12h,压力值为:0kPa,50kPa,100kPa,150kPa和200kPa,应用Westernblot和实时荧光定量RT-PCR检测压力状态下PRG4蛋白和mRNA表达的改变。结果:经单因素方差分析,压力施加后细胞合成PRG4蛋白和mRNA水平均有差异。PRG4蛋白在50kPa和100kPa组表达虽有增高,但与对照组无统计学差异(P>0.05),150kPa和200kPa组表达比对照组显著升高,有统计学差异(P<0.05)。PRG4mRNA的表达与蛋白表达同步。结论:适当的压力负荷可刺激滑膜细胞转录、合成PRG4,压力传递机制与关节润滑机制有密切关联。Objective:The aim of this study was to detect the expression of proteoglycan 4(PRG4)protein and mRNA in the rat synovial fibroblasts under hydrostatic pressure (HP). Methods: The rat temporomandibular joint synovial fibroblasts were primary culture and identified. HP was applied on synovial fibroblasts at the levels of 0kPa, 50kPa, 100kPa, 150 and 200kPa for 12 hours. PRG4 protein and mRNA in different HP were detected by Western blot and real-time PCR. Result: The expressions of PRG4 protein and mRNA were ...

关 键 词：蛋白多糖4 滑膜细胞 压力 

分 类 号：R780.2[医药卫生—口腔医学]