Analysis of nucleolar pre-rRNA processing sites in pea(Pisum sativum)  

作　　者：龙鸿 曾宪录 焦明大 胡波 孙海晶 刘振兰 张立勇 郝水 

机构地区：[1]Institute of Genetics and Cytology,Northeast Normal University

出　　处：《Science China(Life Sciences)》2003年第1期58-66,共9页中国科学（生命科学英文版）

基　　金：the Major State Basic Research Program (973) of China.

摘　　要：The location of rRNA processing was analyzed by using in situ hybridization with ITS1 probe and immunolabeling of anti-fibrillarin mAb in pea (Pisum sativum) root pole cells. The results showed that rRNA processing sites were in dense fibrillar components (DFCs) and granular com-ponents (GCs), but not in fibrillar centers (FCs). Low doses of actinomycin D (AMD) treatment can selectively suppress pre-rRNA synthesis but cannot disturb the processing of preformed pre-rRNAs. With AMD treatment prolonged, the density of labeled signals gradually decreased, indicating the preformed pre-rRNAs were gradually processed.The location of rRNA processing was analyzed by using in situ hybridization with ITS1 probe and immunolabeling of anti-fibrillarin mAb in pea (Pisum sativum) root pole cells. The results showed that rRNA processing sites were in dense fibrillar components (DFCs) and granular com-ponents (GCs), but not in fibrillar centers (FCs). Low doses of actinomycin D (AMD) treatment can selectively suppress pre-rRNA synthesis but cannot disturb the processing of preformed pre-rRNAs. With AMD treatment prolonged, the density of labeled signals gradually decreased, indicating the preformed pre-rRNAs were gradually processed.

关 键 词：PISUM sativum  nucleolus  rRNA  processing  fibrillar centres  dense fibrillar components  GRANULAR components. 

分 类 号：S643[农业科学—蔬菜学]