Effects of BaCl_2 on slow vacuolar ion channels on radish by patch-clamp  

作　　者：杨频 张丽平 

机构地区：[1]Institute of Molecular Science, Shanxi University, Taiyuan 030006, China

出　　处：《Science China Chemistry》2003年第1期84-89,共6页中国科学（化学英文版）

基　　金：We thank Dr. Pei Zhenming for technique advice on the patch clamp. This work was supported by the National Natural Science Foundation of China (Grant No. 29890280).

摘　　要：The effects of BaCl_2 on slow vacuolar (SV) currents of radish are studied byusing the whole-vacuolar patch-clamp recording mode. The Ca^(2+)-dependent SV channel can beactivated by cytosolic Ca^(2+). When 1 mmol/L BaCI_2 is added into pipette solution, SV currents aresuppressed remarkably. Then adding BaCI_2 of different concentrations into the bath solution, SVcurrents reflect different effects. The results show that BaCl_2 with a lower concentration (< 3mmol/L) promotes the channel currents and the currents are saturated when BaCl_2 concentrations arebetween 1 μmol/L and 1 mmol/L, but BaCl_2 with higher concentration (≥ 3 mmol/L) inhibits SVcurrents.Effects of BaCl2 on slow vacuolar ion channels on radish by patch-clamp YANG Pin (杨 频) & ZHANG Liping (张丽平) Institute of Molecular Science, Shanxi University, Taiyuan 030006, China Correspondence should be addressed to Yang Pin (email: [e-mail])

关 键 词：patch-clamp  whole-vacuolar recording  SLOW VACUOLAR channel  CYTOSOLIC Ca2+  Ba2+. 

分 类 号：Q945[生物学—植物学]