肿瘤靶向治疗用白蛋白磁性纳米粒子的188铼(188Re)标记  

Labeling HSA-Coated Magnetite Nanoparticals with Rhenium-188 for Tumor Target Therapy

作　　者：冯彦林[1] 谭家驹[1] 张春富[2] 曹金全[2] 曹本洪[2] 尹端[2] 汪勇先[2] 

机构地区：[1]广东省佛山市第一人民医院,广东佛山528000 [2]中国科学院上海原子核研究所,上海201800

出　　处：《中国医学工程》2003年第6期5-8,共4页China Medical Engineering

基　　金：We are thankful for Science(Technology Commission of Shanghai Municipality(STCSM No.0149 nm066)for financial support;Supported Partially by Key Project of Knowledge Innovation Program of Chinese Academy of Sciences(No.KJCX1-SW-08);Hi-Tech R(D Program of China(“863”No.2001AA218011).

摘　　要：目的　188铼 ( 188Re)标记用于肿瘤靶向治疗的白蛋白磁纳米体的实验室条件。方法　　188Re标记白蛋白磁性纳米体的最佳实验室条件是 :SnCl2 ·H2 O 8mg/ml,柠檬酸 2 0mg/ml,维生素C 8mg/ml,反应容积为 5 0 0 μl ,反应时间为 3h。结果　188Re总的标记率大于 90 % ,标记稳定性 7h为 98%、2 4h为 95 %。结论　188Re-白蛋白磁性纳米体的标记率及标记稳定性适于用于肿瘤靶向治疗的体内研究。Objective:To prepare HSA-coated magnetite nanoparticles labeled with rhenium-188( 188 Re) for the purpose of regional target therapy.Methods: The optimum labeling conditions are that: SnCl 2·H 2O 8?mg/ml, citric acid 20?mg/ml, Vc 8?mg/ml, reaction volume 500?μl and reaction time 3?hours. Results: The labeling efficiency is about 90%. The labeling stability ( 37℃ in PBS at pH 7.4) is about 98% rhenium-188 is still bound 7?h later, 95% 24?h later.Conclusions: The efficiency and stability of labeling HAS-coated magnetite nanoparticles with 188 Re is suitable for in vivo study.

关 键 词：188铼 标记 磁纳米粒子 白蛋白 靶向治疗 

分 类 号：R735.7[医药卫生—肿瘤]