靶向治疗用Fe304及其白蛋包被磁性纳米粒子的制备  

Preparation Fe_3O_4 Nanoparticles and HSA-Coated Magnetite Nanoparticals for Tumor Target Therapy

作　　者：谭家驹[1] 张春富[2] 冯彦林[1] 曹金全[2] 曹本洪[2] 尹端[2] 汪勇先[2] 

机构地区：[1]广东省佛山市第一人民医院,广东佛山528000 [2]中国科学院上海原子核研究所,上海201800

出　　处：《中国医学工程》2003年第6期30-32,共3页China Medical Engineering

基　　金：Science(Technology Commission of Shanghai Municipality(STCSM No.0149nm066);Knowledge Innovation Program of Chinese Academy of Sciences(No.KJCX1-SW-08);Hi-Tech R&D Program of China(“863”No.2001AA218011).

摘　　要：目的　制备用于肿瘤靶向治疗的Fe3 O4及其白蛋白包被的磁性纳米粒子。方法　采用部分还原法制备Fe3 O4纳米粒子 ,通过微乳化方法制备了白蛋白包被的Fe3 O4磁性纳米颗粒。结果　Fe3 O4粒径为 10nm左右 ,X -射线粉末衍射分析显示Fe3 O4纳米磁性微粒是典型的尖晶石构型 ;白蛋白包被的磁性纳米粒子直径在 2 0 0nm左右。结论　Fe3Objective:To prepare Fe 3O 4 magnetite nanoparticles and HSA-coated magnetite particles for the purpose of regional target therapy. Methods: To adopt partial reduction method to prepare the Fe 3O 4 nanoparticles: 100?ml 0.01?mol/L Na 2SO 3 was added dropwise into 100?ml 0.06?mol/LFeCl 3 solution under nitrogen gas flow. 10%(V%) ammonia was added dropwise with rapid stirring until pH of the reaction solution reach 8. Heated at 70℃ with water bath for 15?min. Preparation of HAS-coated magnetite particles:To use microemulsion approach, with oleic acid as oil phase, mixture of HSA and magnetite solution as water phase and sp-85 as emulsion agent.Results: Fe 3O 4 magnetite particles with about 10?nm in diameter and X-ray power diffraction show that the nanoparticles is γ-Fe 2O 3. HAS-coated Fe 3O 4 magnetite nanoparticles is characterized by TEM with diameter no more than 200?nm.Conclusions: Fe 3O 4 magnetite nanoparticles and HSA-coated magnetite particles is suitable for researching of regional target therapy.

关 键 词：靶向治疗 磁性纳米粒子 白蛋白包被 制备 

分 类 号：R735.7[医药卫生—肿瘤]