茶多酚体内外抗流感病毒作用研究  

Study on Anti-influenza Virus Effect of Tea Polyphenols in vitro and in vivo

作　　者：黄深惠[1] 汤有志[1] 周雪梦[1] 谢果[2] 栗原博[2] 何子华[1] 陈建新[1] 

机构地区：[1]华南农业大学兽医学院广东省兽药研制与安全评价重点实验室,广东广州510640 [2]暨南大学药学院中药及天然药物研究所,广东广州510620

出　　处：《茶叶科学》2010年第4期302-308,共7页Journal of Tea Science

基　　金：国家自然科学基金项目(30972217);广东省教育部产学研结合项目(2009B090300331)

摘　　要：研究了没食子儿茶素(Gallocatechin,GC)、表儿茶素(Epicatechin,EC)、表没食子儿茶素(Epigallocatechin,EGC)、表儿茶素没食子酸酯(Epicatechin gallate,ECG)、表没食子儿茶素没食子酸酯(Epigallocatechin gallate,EGCG)、原儿茶醛(Protocatechuic aldehyde,PAD)、原儿茶酸(Protocatechuic acid,PA)共7种茶多酚类物质在MDCK细胞中的抑制流感病毒活性。结果表明,EGCG和ECG具有显著的抑制病毒作用,对感染H5N1、H1N1和H9N23种亚型流感病毒的MDCK细胞50%保护率浓度(EC50)分别在0.04~0.11mmol/L和0.05~0.07mmol/L范围,其保护效果均优于阳性对照药利巴韦林(EC50:0.41~0.53mmol/L)。7种茶多酚类物质对流感病毒神经氨酸酶(NA)均有不同程度的抑制作用,EGCG和ECG对H5N1、H1N1和H9N23种亚型流感病毒NA活性抑制浓度(IC50)分别在0.03~0.14mmol/L和0.34~0.69mmol/L范围。茶...Tea polyphenols gallocatechin(GC),epicatechin(EC),epigallocatechin(EGC),epicatechin gallate(ECG),epigallocatechin gallate(EGCG),protocatechuic aldehyde(PAD) and protocatechuic acid(PA) were evaluated for their ability to inhibit influenza virus in MDCK cells.Among these compounds,the EGCG and ECG showed a marked antiviral effect against influenza virus infections in MDCK cells and this effect was observed in all influenza virus subtypes tested,including H5N1,H1N1 and H9N2 virus.The 50% effective inhibitory ...

关 键 词：茶多酚 流感病毒 神经氨酸酶 小鼠 肺炎 MDCK细胞 

分 类 号：R285[医药卫生—中药学]