Nuclear reconstitution of demembranated Orychophragmus violaceus sperm in Xenopus laevis egg extracts  

作　　者：卢萍 任民 翟中和 

出　　处：《Science China(Life Sciences)》2002年第6期623-630,共8页中国科学（生命科学英文版）

基　　金：This work was supported by the National Natural Science Foundation of China (Grant Nos. 19890380 & 30070388) ; the Special Funds for Major State Basic Research of China

摘　　要：The cell-free extracts from animal Xenopus laevis egg could induce chromatin decon- densation and pronuclear formation from demembranated plant (Orychophragmus violaceus)sperm. The demembranated Orychophragmus violaceus sperm began to swell in 30 min incubation, and then were gradually decondensed. The reassembly of nuclear envelope in the reconstituted nuclei had been visualized by means of electron microscopy and fluorescent microscopy. Membrane vesicles fused to form the double envelope around the periphery of the decondensed chromatin. The morphology of the newly formed nucleus, with a double membrane, was similar to those nuclei after fertilization. Transmission electron microscope micrograph of the whole mount prepared nuclear matrix-lamina showed the reconstituted nucleus to be filled with a dense network.The cell-free extracts from animal Xenopus laevis egg could induce chromatin decon-densation and pronuclear formation from demembranated plant (Orychophragmus violaceus) sperm. The demembranated Orychophragmus violaceus sperm began to swell in 30 min incubation, and then were gradually decondensed. The reassembly of nuclear envelope in the reconstituted nuclei had been visualized by means of electron microscopy and fluorescent microscopy. Membrane vesicles fused to form the double envelope around the periphery of the decondensed chromatin. The morphology of the newly formed nucleus, with a double membrane, was similar to those nuclei after fertilization. Transmission electron microscope micrograph of the whole mount prepared nuclear matrix-lamina showed the reconstituted nucleus to be filled with a dense network.

关 键 词：CELL-FREE system  nuclear reconstitution  Orychophragmus violaceus  demembranated sperm  XENOPUS laevis  egg extracts. 

分 类 号：Q253[生物学—细胞生物学]