Isolation of Protoplast and Ion Channel Recording in Plasma Membrane of Suspension Cells of Populus euphratica  

胡杨悬浮细胞原生质体游离及质膜单离子通道测定(英文)

作　　者：陈少良[1] 戴松香[1] 李金克[1] 王沙生[1] Andrea Polle Aloys Hüttermann 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083 [2]哥廷根大学森林植物研究所

出　　处：《Forestry Studies in China》2002年第1期1-4,共4页中国林学（英文版）

基　　金：SupportedinpartbytheNationalNaturalScienceFoundationofChina(NNSF) (GrantNo .30 0 70 6 13) ;theFoundationfortheAuthorsofNationalExcellentDoctoralDissertationofP .R .China(GrantNo .2 0 0 15 2 ) ;Nation alLaboratoryofBiomembranesandMembraneBiotech

摘　　要：The authors used suspension cells of Populus euphratica to isolate protoplast in the present study. Protoplasts were successfully obtained after 4 hours incubation in enzyme solution containing 1 0% cellulase “onozuka” R\|10, 0\^01% pectolyase Y\|23,0\^15% macerozyme R\|10 and 0\^1% hemicellulase at 25℃. Outward and inward single channels in plasma membrane were observed using cell\|attached recording of patch\|clamp technique. In this study, single channel records showed that more than one species of channel were obtained. These attempts in protoplast isolation and ion channel recording offers the opportunity to characterize cellular mechanisms of salt tolerance in tree species.The authors used suspension cells of Populus euphratica to isolate protoplast in the present study. Protoplasts were successfully obtained after 4 hours incubation in enzyme solution containing 1 0% cellulase “onozuka” R\|10, 0\^01% pectolyase Y\|23,0\^15% macerozyme R\|10 and 0\^1% hemicellulase at 25℃. Outward and inward single channels in plasma membrane were observed using cell\|attached recording of patch\|clamp technique. In this study, single channel records showed that more than one species of channel were obtained. These attempts in protoplast isolation and ion channel recording offers the opportunity to characterize cellular mechanisms of salt tolerance in tree species.

关 键 词：Populus euphratica suspension cells PROTOPLASTS patch\|clamp cell\|attached recording technique single ion channel 

分 类 号：Q943.1[生物学—植物学]