云南高校图书馆联盟文献共享服务平台- PROTOPLASTS

PROTOPLASTS: 作品数：62被引量：74H指数：4; 导出分析报告; 相关领域：生物学农业科学更多>>; 相关作者：洪建中周东坡平文祥茹炳根张竞更多>>; 相关机构：齐齐哈尔师范学院武汉大学北京大学北京林业大学更多>>; 相关期刊：《Journal of Ocean University of China》《Science China(Life Sciences)》《Science China Chemistry》《Developmental and Reproductive Biology》更多>>; 相关基金：国家自然科学基金中国科学院知识创新工程教育部跨世纪优秀人才培养计划更多>>

Optical ratiometric silica pH sensors unveil active transport and subcellular particle localization in protoplasts: 《Nano Research》2025年第3期444-457,共14页Helena Iuele Lorenzo Maria Curci Alessio Bucciarelli Valentina Onesto Stefania Forciniti Giuseppe Gigli Loretta L.del Mercato Monica De Caroli Gabriella Piro; supported by the European Research Council(ERC)under the European Union’s Horizon 2020 research and innovation program ERC Starting Grant“INTERCELLMED”(Nr.759959);the Associazione Italiana per la Ricerca contro il Cancro(AIRC)(MFAG-2019,Nr.22902);the PRIN 2022(Nr.2022CRFNCP_PE11_PRIN2022 and PRIN 2022-SAPPHIRE(CUP 2022MWK7Y7);funded by European Union-Next Generation EU,the“Tecnopolo per la medicina di precisione”(TecnoMed Puglia)-Regione Puglia:DGR n.2117 of 21/11/2018,CUP B84I18000540002);the Italian Ministry of Research(MUR)in the framework of the National Recovery and Resilience Plan(NRRP),“NFFA-DI”Grant(CUP B53C22004310006);“I-PHOQS”Grant(CUP B53C22001750006);under the complementary actions to the NRRP funded by NextGenerationEU,“Fit4MedRob”Grant(PNC0000007,CUP B53C22006960001).; Understanding the interaction mechanisms of engineered nanomaterials(ENMs)with plant membranes is crucial for their effective use in various applications.While passive transport of smaller ENMs is well-documented,the ...; 关键词：optical ratiometric pH-sensors intracellular pH tracking PROTOPLAST endocytic pathway computational analysis

Construction and optimization of a genetic transformation system for efficient expression of human insulin-GFP fusion gene in flax: 《Bioresources and Bioprocessing》2024年第1期1102-1114,共13页Wei Zhao Rui Zhang Luyang Zhou Zhongxia Zhang Fei Du Ruoyu Wu Jing Kong Shengjun An; funded by Hebei Development and Reform Commission of China,Hebei Engineering Laboratory,grant number(2021)1157 and the Innovation Fund Project of Hebei University of Engineering(114/SJ2401002149).; The human insulin gene modified with a C-peptide was synthesized according to the plant-preferred codon,and a fusion gene expression vector of insulin combined with green fluorescent protein(GFP)was constructed.The op...; 关键词：Flax callus Insulin-GFP Genetic transformation Agrobacterium-induced infection PROTOPLASTS

An efficient transient gene expression system for protein subcellular localization assay and genome editing in citrus protoplasts被引量：2: 《Horticultural Plant Journal》2023年第3期425-436,共12页Wenhui Yang Jiaqin Ren Wanrong Liu Dan Liu Kaidong Xie Fei Zhang Pengwei Wang Wenwu Guo Xiaomeng Wu; supported by the National Natural Science Foundation of China;China (Grant Nos. 31872051, 32072528);the Foundation of Hubei Hongshan Laboratory (Grant No.2021hszd009)。; Protoplast has been widely used in biotechnologies to circumvent the breeding obstacles in citrus, including long juvenility, polyembryony, and male/female sterility. The protoplast-based transient gene expression sys...; 关键词：CITRUS Callus protoplast Transient transfection Subcellular localization Genome editing

Escherichia coli Protoplast Fusion and Screening of High L-Lysine-Producing Strain: 《Agricultural Biotechnology》2022年第3期127-132,135,共7页Zirui WANG Junqing WANG; Supported by the Focus on Research and Development Plan in Shandong Province(2019JZZY011003,2020CXGC010603);National Natural Science Foundation of China(31801527)。; [Objectives]Protoplast fusion of two parental strains with weak L-lysine production ability was carried out to obtain new fusion strains with strong L-lysine production ability.[Methods]The effects of bacterial age,ly...; 关键词：Escherichia coli L-LYSINE PROTOPLASTS Cell fusion Microbial breeding

Development of potent promoters that drive the efficient expression of genes in apple protoplasts被引量：2: 《Horticulture Research》2021年第1期2872-2880,共9页Xianpu Wang Lili Xu Xiuxia Liu Li Xin Shujing Wu Xuesen Chen; This work was supported by grants from the National Key Research and Development Program of China(2018YFD1000307);the Natural Science Foundation in China(nos.31672136 and 31272132).; Protoplast transient expression is a powerful strategy for gene functional characterization,especially in biochemical mechanism studies.We herein developed a highly efficient transient expression system for apple prot...; 关键词：herein TRANSIENT stability

Loading calcium fluorescent probes into protoplasts to detect calcium in the flesh tissue cells of Malus domestica: 《Horticulture Research》2020年第1期1561-1571,共11页Lina Qiu Yongzhang Wang Haiyong Qu; supported by the National Key Research and Development Plan Project:Integrated research and demonstration on the technology of reducing application and increasing efficiency of chemical fertilizer and pesticide in apple cultivation(2016YFD0201120).; Cytosolic Ca^(2+)plays a key role in signal transduction in plants.Calcium imaging is the most common approach to studying dynamic changes in the cytoplasmic Ca^(2+)content.Here,we used mature‘Fuji’apples(Malus pumi...; 关键词：reversed probes HYDROLYSIS

Construction of PEG-mediated Genetic Transformation and Gene Knockout System in Fusarium oxysporum f. sp.cubense Tropic Race 4被引量：1: 《Agricultural Biotechnology》2020年第1期15-17,21,共4页Lei ZHANG Yan GUO Yunyue WANG Weihua TANG Sijun ZHENG; Supported by Yunnan Science and Technology Innovation Talent Program(2015HA034);National Natural Science Foundation of China(NSFC31560505); Fusarium wilt of banana, caused by Fusarium oxysporum f. sp. cubense tropic race 4(Foc TR4), is a typical vascular and soil-borne disease which has significantly threatened the sustainable development of banana indust...; 关键词：Fusarium wilt of banana Fusarium oxysporum f.sp.cubense PROTOPLASTS Transformation Split-marker recombination

Isolation and callus formation of Gracilariopsis bailiniae(Gracilariales, Rhodophyta) protoplasts: 《Journal of Oceanology and Limnology》2018年第6期2268-2277,共10页CHEN Haihong CHEN Weizhou SHI Jingyi CHEN Zepan ZHANG Yi; Supported by the China Agriculture Research System(No.CARS-50);the Science and Technology Program of Guangdong Province of China(Nos.2016A020222023,2015B090903081);the Project of Guangdong Province Education Department(No.2017KCXTD014); This paper reports the first successful isolation of protoplasts from G racilariopsis bailiniae and their callus formation. The base solution type, concentration of isolating enzymes, concentration of sorbitol, incuba...; 关键词：GRACILARIOPSIS bailiniae enzyme marine bacterium PROTOPLAST ISOLATION cell division CALLUS

Gene delivery into isolated Arabidopsis thaliana protoplasts and intact leaves using cationic, a-helical polypeptide被引量：2: 《Frontiers of Chemical Science and Engineering》2017年第4期521-528,共8页; The application of gene delivery materials has been mainly focused on mammalian cells while rarely extended to plant engineering. Cationic polymers and lipids have been widely utilized to efficiently deliver DNA and s...; 关键词：a-helical polypeptide plant gene delivery protoplast intact leaves TRANSFECTION

Purified isolation of vacuoles from Sedum alfredii leaf-derived protoplasts被引量：1: 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2017年第1期85-88,共4页Xiao-yu GAO Xing-cheng LIAO Ruo-lai WU Ting LIU Hai-xing WANG Ling-li LU; Project supported by the National Natural Science Foundation of China(Nos.31370040 and 41401366);the Zhejiang Provincial Natural Science Foundation of China(No.LR14C150001); This study aims to develop a method for isolating and purifying protoplasts/vacuoles from fresh leaves of the Cd hyperaccumulator plant species, Sedum alfredii. The results revealed that preheating cellulase and macer...; 关键词：超积累植物东南景天原生质体液泡提取与纯化

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