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PROTOPLAST: 作品数：84被引量：170H指数：6; 导出分析报告; 相关作者：茹炳根张竞单龙聂思惟王沙生更多>>; 相关机构：成都学院(成都大学)四川抗菌素工业研究所北京大学北京林业大学中国农业科学院植物保护研究所更多>>; 相关期刊：更多>>; 相关基金：国家自然科学基金广东省自然科学基金国家高技术研究发展计划国家重点基础研究发展计划更多>>

Optical ratiometric silica pH sensors unveil active transport and subcellular particle localization in protoplasts: 《Nano Research》2025年第3期444-457,共14页Helena Iuele Lorenzo Maria Curci Alessio Bucciarelli Valentina Onesto Stefania Forciniti Giuseppe Gigli Loretta L.del Mercato Monica De Caroli Gabriella Piro; supported by the European Research Council(ERC)under the European Union’s Horizon 2020 research and innovation program ERC Starting Grant“INTERCELLMED”(Nr.759959);the Associazione Italiana per la Ricerca contro il Cancro(AIRC)(MFAG-2019,Nr.22902);the PRIN 2022(Nr.2022CRFNCP_PE11_PRIN2022 and PRIN 2022-SAPPHIRE(CUP 2022MWK7Y7);funded by European Union-Next Generation EU,the“Tecnopolo per la medicina di precisione”(TecnoMed Puglia)-Regione Puglia:DGR n.2117 of 21/11/2018,CUP B84I18000540002);the Italian Ministry of Research(MUR)in the framework of the National Recovery and Resilience Plan(NRRP),“NFFA-DI”Grant(CUP B53C22004310006);“I-PHOQS”Grant(CUP B53C22001750006);under the complementary actions to the NRRP funded by NextGenerationEU,“Fit4MedRob”Grant(PNC0000007,CUP B53C22006960001).; Understanding the interaction mechanisms of engineered nanomaterials(ENMs)with plant membranes is crucial for their effective use in various applications.While passive transport of smaller ENMs is well-documented,the ...; 关键词：optical ratiometric pH-sensors intracellular pH tracking PROTOPLAST endocytic pathway computational analysis

An efficient and universal protoplast-based transient gene expression system for genome editing in Brassica crops: 《Horticultural Plant Journal》2024年第4期983-994,共12页Xiaoxiao Yu Jintai Yu Yin Lu Wenjing Li Guanzhong Huo Jun Zhang Yan Li Jianjun Zhao Jun Li; financially supported by the Key project of National Natural Science Foundation of China (Grant No.32330096);Innovative Research Group Project of Hebei Natural Science Foundation (Grant No.C2024204246);S&T Program of Hebei (Grant Nos.21372901D;23567601H);Natural Science Foundation of Hebei (Grant No.C2023204119);the Starting Grant from Hebei Agricultural University (Grant No.YJ201958)。; Protoplast-based transient gene expression system has been widely used in plant genome editing because of its simple operation and less time-consuming.In order to establish a universal protoplast-based transient trans...; 关键词：Chinese cabbage PTGE Transfection efficiency CRISPR/Cas9

Optimized protoplast isolation and transfection with a breakpoint:accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot: 《aBIOTECH》2024年第2期151-168,共18页Debasmita Panda Subhasis Karmakar Manaswini Dash Swagat Kumar Tripathy Priya Das Sagar Banerjee Yiping Qi Sanghamitra Samantaray Pradipta Kumar Mohapatra Mirza J.Baig Kutubuddin A.Molla; support from the University Grant Commission(UGC);funding support from the Science and Engineering Research Board(SERB)-National Post-Doctoral Fellowship.;funding support from the Department of Biotechnology(DBT),Government of India-JRF program.MD,SB,MJB;funding from the Indian Council of Agricultural Research(ICAR),New Delhi,in the form of the Plan Scheme-‘Incentivizing Research in Agriculture’project;support from the Director,NRRI.; The CRISPR-Cas genome editing tools are revolutionizing agriculture and basic biology with their simplicity and precision ability to modify target genomic loci.Software-predicted guide RNAs(gRNAs)often fail to induce ...; 关键词：PROTOPLAST Genome editing Transgene-free edited plants Rice CHICKPEA

Role of phosphorus on the biogeochemical behavior of cadmium in the contaminated soil under leaching and pot experiments: 《Journal of Environmental Sciences》2024年第3期488-499,共12页Hui Jia YifanWu Mengqi Zhang Jinhui Ye Daolin Du He Wang; supported by the Natural Science Foundation of Jiangsu Province (No.BK20210751);China Postdoctoral Research Foundation (No.2022M711389);the Carbon Peak and Carbon Neutrality Technology Innovation Foundation of Jiangsu Province (No.BK20220030)。; Phosphorus(P)is involved in various biochemical reactions in plant growth,so it is beneficial to plants growing in soils contaminated by metals,including cadmium(Cd).However,few studies have reported on the mechanisti...; 关键词：PHOSPHORUS Cadmium stress PROTOPLAST Biogeochemical behavior Mitochondrial function

A cyclic effect of cAMP and calcium signaling contributes to jujube growth and development: 《Journal of Integrative Agriculture》2023年第7期2094-2110,共17页WANG Li-xin WANG Lin-xia ZHANG Meng-ling QU Ying-yue YUAN Ye Ehsan SADEGHNEZHAD GAO Meng-jiao ZHAO Ruo-yu QI Chao-feng GUO Xiao-xue ZHU Wen-hui LI Rui-mei DAI Li LIU Meng-jun LIU Zhi-guo; This research was funded by the Provincial Supporting Program of Hebei for the Returned Oversea Scholars,China(C20210114);the Science and Technology Project of Hebei Education Department(QN2022017);the Fundamental Scientific Research Fund of Universities in Hebei Province(KY2021059);the China Agriculture Research System(CARS-30-2-07);the National Key Research and Development Project of China(2019YFD1001605);the Natural Science Foundation of Hebei Province(C2020204082);the Funds for Hebei Jujube Industry Technology Research Institute after Operation Performance(205676155H);the Young Talent Project of Hebei Agricultural University Foundation(YJ201853).; 3´,5´-Cyclic adenosine monophosphate(cAMP)is an important metabolite that is specifically enriched in jujube.However,the effect of cAMP on jujube cellular responses has not been comprehensively studied.Here,we estab...; 关键词：CAMP CALCIUM growth suspension cells PROTOPLAST signaling JUJUBE

An efficient transient gene expression system for protein subcellular localization assay and genome editing in citrus protoplasts被引量：2: 《Horticultural Plant Journal》2023年第3期425-436,共12页Wenhui Yang Jiaqin Ren Wanrong Liu Dan Liu Kaidong Xie Fei Zhang Pengwei Wang Wenwu Guo Xiaomeng Wu; supported by the National Natural Science Foundation of China;China (Grant Nos. 31872051, 32072528);the Foundation of Hubei Hongshan Laboratory (Grant No.2021hszd009)。; Protoplast has been widely used in biotechnologies to circumvent the breeding obstacles in citrus, including long juvenility, polyembryony, and male/female sterility. The protoplast-based transient gene expression sys...; 关键词：CITRUS Callus protoplast Transient transfection Subcellular localization Genome editing

The protoplast two-way fusions and fusant characteristics of Antrodia cinnamomea and Cordyceps militaris被引量：1: 《Food Science and Human Wellness》2022年第5期1240-1251,共12页Wei-Kuang Lai Yang-Chang Wu Tai-Sheng Yeh Chun-Ren Hsieh Yi-Hong Tsai Chien-Kei Wei Chi-Ying Li Ying-Chen Lu Fang-Rong Chang; supported by grants from the Ministry of Science and Technology of Taiwan(Grant number:MOST 106-2320-B-037008-MY2,MOST 108-2320-B-037-022-MY3,108-2811-B-037-511,and 109-2927-I-037-502);funded by the Drug Development and Value Creation Research Center,Kaohsiung Medical University;Department of Medical Research,Kaohsiung Medical University Hospital(Grant number:KMU-TC108A03-11)。; This study generated two fused protoplasts of Antrodia cinnamomea and Cordyceps militaris in two ways.The protoplasts of A.cinnamomea were inactivated by heat to inactivate biochemical processes and enzymatic activiti...; 关键词：Medicinal fungi Protoplast fusion Antrodia cinnamomea Cordyceps militaris Secondary metabolite

Research Progress of Algae Protoplast Fusion Technique: 《Asian Agricultural Research》2022年第2期39-40,共2页Xiaoying XU Wenkai SHI Lei LI Ya'nan CAO Xiaoguang LU Peng LIU; This paper discussed the technique of algal protoplast fusion,including the method of protoplast-induced fusion,the screening and identification of hybrid cells and so on.In this paper,the research progress of protopl...; 关键词：ALGAE Protoplast fusion Research progress

Strong Allelopathic Activities of Leaves and Cultured Cells of Spiraea thunbergiiAssayed by the Protoplast Co-Culture Method with Digital Image Analysis: Evaluation of Cis- and Trans-Cinnamic Acid as Allelochemicals: 《American Journal of Plant Sciences》2021年第11期1673-1690,共18页Sakae Suzuki Mari Kimura Rei Yasuda Naoya Wasano Yutaka Sasamoto Yoshiharu Fujii Hamako Sasamoto; The inhibitory allelopathic activities of leaves and leaf-origin suspension cultured cells of Spiraea thunbergii, and putative allelochemicals, cis- and trans-cinnamic acid, were investigat...; 关键词：ALLELOPATHY Cinnamic Acid Protoplast Co-Culture Spiraea thunbergii

Evaluation of Isoflavones as Allelochemicals with Strong Allelopathic Activities of Kudzu Using Protoplast Co-Culture Method with Digital Image Analysis被引量：1: 《American Journal of Plant Sciences》2021年第3期376-393,共18页Kentaro Kobayashi Hamako Sasamoto Yutaka Sasamoto Akifumi Sugiyama Yoshiharu Fujii; The inhibitory allelopathic activity of Pueraria montana (Kudzu), and activities of two putative allelochemical isoflav...; 关键词：Allelopathy Bioassay DAIDZEIN ISOFLAVONE Protoplast Culture PUERARIN

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