A cyclic effect of cAMP and calcium signaling contributes to jujube growth and development  

作　　者：WANG Li-xin WANG Lin-xia ZHANG Meng-ling QU Ying-yue YUAN Ye Ehsan SADEGHNEZHAD GAO Meng-jiao ZHAO Ruo-yu QI Chao-feng GUO Xiao-xue ZHU Wen-hui LI Rui-mei DAI Li LIU Meng-jun LIU Zhi-guo 

机构地区：[1]College of Horticulture,Hebei Agricultural University,Baoding 071001,P.R.China [2]Research Center of Chinese Jujube,Hebei Agricultural University,Baoding 071001,P.R.China [3]Key Laboratory of Resource Biology and Biotechnology in Western China,Ministry of Education/College of Life Sciences,Northwest University,Xi’an 710069,P.R.China [4]Department of Plant Biology,Faculty of Biological Sciences,Tarbiat Modares University,Teheran 14115,Iran

出　　处：《Journal of Integrative Agriculture》2023年第7期2094-2110,共17页农业科学学报（英文版）

基　　金：This research was funded by the Provincial Supporting Program of Hebei for the Returned Oversea Scholars,China(C20210114);the Science and Technology Project of Hebei Education Department(QN2022017);the Fundamental Scientific Research Fund of Universities in Hebei Province(KY2021059);the China Agriculture Research System(CARS-30-2-07);the National Key Research and Development Project of China(2019YFD1001605);the Natural Science Foundation of Hebei Province(C2020204082);the Funds for Hebei Jujube Industry Technology Research Institute after Operation Performance(205676155H);the Young Talent Project of Hebei Agricultural University Foundation(YJ201853).

摘　　要：3´,5´-Cyclic adenosine monophosphate(cAMP)is an important metabolite that is specifically enriched in jujube.However,the effect of cAMP on jujube cellular responses has not been comprehensively studied.Here,we established jujube cell suspension cultures and investigated the calcium influx in response to cAMP treatment through protoplast isolation and fluorescence intensity.Firstly,cAMP treatment could promote jujube growth and increase the content of endogenous cAMP.Using transcriptome analysis with transgenic Arabidopsis plants overexpressing adenylate cyclase(ZjAC)as a positive control,we identified 60 calcium-related differential expressed genes(DEGs)that contributed to the calcium signaling and inter-or intra-cellular responses.Pharmacological treatments such as cAMP and the calcium ionophore A23187 could induce ZjAC expression,the accumulation of cAMP and calcium influx in jujube cells,while ethylene glycol tetraacetic acid(EGTA)or bithionol treatment inhibited these changes.Moreover,the calcium channels and transporters in calcium influx,such as the ZjCNGC2 channel and the mitogen activated protein(MAP)kinase pathway,could be activated by cAMP treatment.In summary,our findings demonstrated that cAMP biosynthesis is dependent on calcium influx and the amplifying effect between calcium and cAMP may be involved in intracellular signal induction,which might contribute to the growth and development of jujube.

关 键 词：CAMP CALCIUM growth suspension cells PROTOPLAST signaling JUJUBE 

分 类 号：S665.1[农业科学—果树学]