STUDY OF MUTAGENICITY OF VIRAL VECTOR IN TUMOR GENETHERAPY  

作　　者：李贺书 李殿俊 刘旭 李大林 

机构地区：[1]Cancer Research Institute, Harbin 150040 [2]The Third Affiliated Hospital, Harbin Medical University, Harbin 150040

出　　处：《Chinese Journal of Cancer Research》2002年第1期24-27,共4页中国癌症研究（英文版）

基　　金：Foundation item: This work was supported by a grant from the Natural Science Foundation of Heilongjiang Province (D0122).

摘　　要：Objective: To observe the mutagenicity of retrovirus and adenovirus as transgenic vectors to evaluate the safety of transgenic tumor cells as tumor vaccines. Methods: Cells were cultured together with the virus. Then DNA and supernatant were tested for mutagenicity by means of genetic toxicological laboratory technique. Results: The results indicated that DNA and supernatant of transgenic cells had no mutagenicity through both in vivo and in vitro tests. Conclusion: The modified virus had no mutagenicity as a transgenic vector.Objective: To observe the mutagenicity of retrovirus and adenovirus as transgenic vectors to evaluate the safety of transgenic tumor cells as tumor vaccines. Methods: Cells were cultured together with the virus. Then DNA and supernatant were tested for mutagenicity by means of genetic toxicological laboratory technique. Results: The results indicated that DNA and supernatant of transgenic cells had no mutagenicity through both in vivo and in vitro tests. Conclusion: The modified virus had no mutagenicity as a transgenic vector.

关 键 词：Retrovirus adenovirus Tumor genetherapy Ames micronucleus 

分 类 号：R730.54[医药卫生—肿瘤]