Transgenic tobacco plants harboring tomato proteinase inhibitor II gene and their insect resistance  

作　　者：XIE Xianzhi, HUANG Meijuan and WU Naihu( Institute of Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China College of Life Sciences, Peking University, Beijing 100871, China) 

出　　处：《Progress in Natural Science:Materials International》2002年第3期198-203,共6页自然科学进展·国际材料（英文版）

基　　金：Supported by the Langqidao Company, Fuzhou Municipality, Fujian Province

摘　　要：The plant expression vectors pBCT2 and pBT2 were constructed with the cDNA sequence (tin2) and genomic DNA sequence (tin2i) of tomato proteinase inhibitor II gene respectively. Then the two expression vectors were transferred into tobacco via the Agrobacterium tumefaciens strain LBA4404, and transgenic tobacco plants were generated. Molecular analysis and trypsin activity assay showed that both cDNA and genomic DNA were expressed properly in the transgenic plants. Insecticidal activities in these transgenic plants indicated that transgenic tobacco plants carrying tin2i sequence were more resistant to 2-instar larvae of Heliothis armigera Hubner than those carrying tin2 sequence. Therefore the intron of tin2i sequence might be a contributor to insecticidal activity of the transgenic tobacco.The plant expression vectors pBCT2 and pBT2 were constructed with the cDNA sequence ( tin2) and genomic DNA sequence (tin2i) of tomato proteinase inhibitor II gene respectively. Then the two expression vectors were transferred into tobacco via the Agrobacterium tumefaciens strain LBA4404, and transgenic tobacco plants were generated. Molecular analysis and trypsin activity assay showed that both cDNA and genomic DNA were expressed properly in the transgenic plants. Insecticidal activities in these transgenic plants indicated that transgenic tobacco plants carrying tin2i sequence were more resistant to 2-instar larvae of Heliothis armigera Hubner than those carrying tin2 sequence. Therefore the intron of tin2i sequence might be a contributor to insecticidal activity of the transgenic tobacco.

关 键 词：TOMATO PROTEINASE INHIBITOR II gene  TRANSGENIC tobacco  insect-resistance. 

分 类 号：S188[农业科学—农业基础科学]