An intracellular toxic protein (Xin) isolated from Xenorhabdus nematophilus strain BJ  

作　　者：PAN Yinghong , JIAN Heng , ZHANG Jie , LIU Zheng, CHEN Zhongyi , YANG Xiufen, YANG Huaiwen and HUANG Dafang( State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China Institute of Biocontrol Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China Institute of Biotechnology Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China.) 

出　　处：《Progress in Natural Science:Materials International》2002年第4期310-312,共3页自然科学进展·国际材料（英文版）

基　　金：Supported by the National Natural Science Foundation of China (Grant No. 30070519)

摘　　要：An intracellular toxic protein, designated Xin and with remarkable inhibitory effect on growth of Helicoverpa armigera, was isolated from X. Nematophilus strain BJ by ion exchange chromatography and hydrophobic interaction chromatography. After placing neonates on the artificial diet containing Xin at concentration of 0 or 3.48μg/g for 7 days, the average weight of insects was 23.50mg and 0.55mg, respectively. The activity of Xin was lost by heating, freeze-drying, digestion with proteases or urea. The Xin molecular weight, over kD, was determined by gel filtration on Superose 12, and there were six component proteins analyzed by SDS-PAGE. The results show that Xin is a high molecular protein complex inhibiting growth of insect pest.An intracellular toxic protein, designated Xin and with remarkable inhibitory effect on growth of Helicozerpa armigera, was isolated from X. nematophilus strain BJ by ion exchange chromatography and hydrophobic interaction chromatography, After placing neonates on the artificial diet containing Xin at concentration of 0 or 3.48 mug/g for 7 days, the average weight of insects was 23.50 mg and 0.55 mg, respectively. The activity of Xin was lost by heating, freeze-drying, digestion with proteases or urea. The Xin molecular weight, over 1000 kD, was determined by gel filtration on Superose 12, and there were six component proteins analyzed by SDS-PAGE. The results show that Xin is a high molecular protein complex inhibiting growth of insect pest.

关 键 词：INTRACELLULAR protein  HELICOVERPA armigera  XENORHABDUS nematophilus  Xin  purification. 

分 类 号：Q26[生物学—细胞生物学]