Reductase activity of human thioredoxin-like protein and its effects on cell apoptosis  

作　　者：JIN Jian, ZHOU Yan, HU Xiaoyan, SHI Ning, HAN Chunyu, XU Caimin, QIANG Boqin,YUAN Jiangang and PENG Xiaozhong(National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences, National Human Genome Center, Beijing 100005, China) 

出　　处：《Progress in Natural Science:Materials International》2002年第9期713-716,共4页自然科学进展·国际材料（英文版）

基　　金：Supported by the National Natural Science Foundation of China (Grant No. 39830070);the Major State Basic Research Development Program of China (G1998051002) ; National High Technology Research and Development Program (2001AA221041)

摘　　要：Thioredoxin is a ubiquitous dithiol oxidoreductase found in many organisms and involved in numerous biochemical processes. Human thioredoxin-like protein (hTRXL) is differentially expressed at different development stages of human fetal cerebrum and belongs to an expanding family of thioredoxins. Recombinant hTRXL and truncated hTRXLs corresponding to the N-terminal (hTRXL-N) and C-terminal (hTRXL-C) domains are expressed and purified. In insulin disulfide reduction assay, both full-length hTRXL and hTRXL-N show reducing activity for the insulin disulfide bonds. As expected, the hTRXL-C failed to reduce insulin. MCF-7 cell stably transfected with hTRXL cDNA exhibits increased sensitivity to apoptosis induced by phorbol myristic acetate (PMA) and ionomysin.Thioredoxin is a ubiquitous dithiol oxidoreductase found in many organisms and involved in numerous biochemical processes. Human thioredoxin-like protein (hTRXL) is differentially expressed at different development stages of human fetal cerebrum and belongs to an expanding family of thioredoxins. Recombinant hTRXL and truncated hTRXLs corresponding to the N-terminal (hTRXL-N) and C-terminal (hTRXL-C) domains are expressed and purified. In insulin disulfide reduction assay, both full-length hTRXL and hTRXL-N show reducing activity for the insulin disulfide bonds. As expected, the hTRXL-C failed to reduce insulin. MCF-7 cell stably transfected with hTRXL cDNA exhibits increased sensitivity to apoptosis induced by phorbol myristic acetate (PMA) and ionomysin.

关 键 词：HUMAN thioredoxin-like gene (hTRXL)  FETAL cerebrum  reduction assay  apoptosis. 

分 类 号：R341[医药卫生—基础医学]