Cloning of two plastid division ftsZ genes from Nicotiana tabacum and their expression in E. coli  

作　　者：WANG Dong KONG Dongdong HU Yong JU Chuanli HE Yikun and SUN Jingsan(1. Department of Biology, Capital Normal University, Beijing 100037, China 2. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China 3. College of Life Sciences, Beijing Normal University, Beijing 100875, China) 

出　　处：《Progress in Natural Science:Materials International》2002年第12期909-914,共6页自然科学进展·国际材料（英文版）

基　　金：the National Natural Science Foundation of China (Grant No. 39970356); the Natural Science Foundation of Beijing (Grant No. 5992003) ; the China Postdoctoral Foundation

摘　　要：Two cDNAs of plastid division gene NtFtsZ1-1 and NtFtsZ1-2 are isolated from Nicotiana tabacum by RT-PCR and rapid amplification cDNA ends (RACE) method. Analysis of the deduced amino acid sequences encoded by NtFtsZ1-1 and NtFtsZ1-2 indicate that these two proteins possess the typical conservative motifs and GTP binding sites existing in all FtsZ proteins. The existence of putative plastid transit peptide in their N-terminal suggests that there are at least two transit-peptide containing FtsZ proteins in higher plants. Phylogenetic analysis based on amino acid sequences of FtsZ proteins also supports this interference. These two NtFtsZ genes demonstrate a similar expression pattern during the plant development, detected by Northern blot. Expression of NtFtsZ1-1 and NtFtsZ1-2 in E.coli interrupts the normal division process of host cells. These results suggest the diverse functions of FtsZ proteins in higher plants.Two cDNAs of plastid division gene NtFtsZ1-1 and NtFtsZ1-2 are isolated from Nicotiana tabacum by RT-PCR and rapid amplification cDNA ends (RACE) method. Analysis of the deduced amino acid sequences encoded by NtFtsZ1-1 and NtFtsZ1-2 indicate that these two proteins possess the typical conservative motifs and GTP binding sites existing in all FtsZ proteins. The existence of putative plastid transit peptide in their N-terminal suggests that there are at least two transit-peptide containing FtsZ proteins in higher plants. Phylogenetic analysis based on amino acid sequences of FtsZ proteins also supports this interference. These two NtFtsZ genes demonstrate a similar expression pattern during the plant development, detected by Northern blot. Expression of NtFtsZ1-1 and NtFtsZ1-2 in E. coli interrupts the normal division process of host cells. These results suggest the diverse functions of FtsZ proteins in higher plants.

关 键 词：NtFtsZ genes  PLASTID division  NICOTIANA tabacum  PLASTID transit peptide  EXPRESSION in E.coli. 

分 类 号：Q785[生物学—分子生物学]