APC和c-Myc在非小细胞肺癌中的表达及意义  

Expressions of APC and c-Myc and Its Implication on Non-small Cell Lung Cancer

作　　者：王世凤[1] 刘倩[2] 张尚福[1] 廖殿英[1] 徐缓[1] 张文燕[1] 李为民[3] 刘伦旭[4] 

机构地区：[1]四川大学华西医院病理科,成都610041 [2]德阳市人民医院病理科,德阳618000 [3]四川大学华西医院呼吸内科,成都610041 [4]四川大学华西医院胸外科,成都610041

出　　处：《四川大学学报（医学版）》2010年第5期822-826,共5页Journal of Sichuan University(Medical Sciences)

基　　金：国家"十一.五"科技攻关课题(No.2006BAI02A01)资助

摘　　要：目的利用组织芯片技术探讨结肠腺瘤性息肉病基因产物APC蛋白和c-Myc蛋白在非小细胞肺癌(NSCLC)及其淋巴结转移灶中的表达及意义。方法采用免疫组织化学EliVision法和EnVision法检测270例NSCLC原发灶、55例淋巴结转移灶以及46例癌旁正常肺组织中APC和c-Myc的表达。结果 APC和c-Myc在NSCLC原发灶中的阳性表达率均高于淋巴结转移灶中的表达率,差异有统计学意义(P<0.05)。在NSCLC原发灶中,APC和c-Myc在不同组织学类型、TNM分期及有无淋巴结转移的病例中的表达不同(P均<0.05);在NSCLC组织中APC与c-Myc的表达呈正相关(rs=0.376,P=0.000)。单因素生存分析显示APC与c-Myc阳性表达组的总体生存率均低于阴性表达组(P<0.05)。Cox回归分析提示肿瘤的组织学类型、分化程度、淋巴结转移及c-Myc的表达是影响NSCLC患者预后的独立危险因素(P<0.05)。结论 APC和c-Myc在NSCLC的发生、发展中具有重要作用。Objective To investigate the expressions of adenomatous polyposis coli(APC) protein and c-Myc protein in non-small cell lung cancer(NSCLC) and their lymph node metastases.Methods APC and c-Myc proteins were detected in 270 cases of primary NSCLC,55 cases of lymph node metastatic tissues and 46 cases of adjacent normal lung tissues by EliVision and EnVision methods of immunohistochemical staining.Results Higher rates of the expressions of both APC and c-Myc proteins in NSCLC primary foci were found compared with those in lymph node metastases(P<0.05).Furthermore,the expressions of APC and c-Myc proteins varied with histological types,TNM stagings and metastasis of the NSCLC(P<0.05).The Spearman correlation analysis showed that the expressions of APC and c-Myc proteins were positively correlated(rs=0.376,P=0.000).The Kaplan-Meier survival analysis revealed that the survival rate was lower in patients with positive expressions of APC and c-Myc proteins than in patients with negative expressions(P<0.05).Histological type,pathologic grading,metastasis and c-Myc were identified as independent risk factors with related to the prognosis of NSCLC patients in the multivariate Cox regression model(P<0.05).Conclusion APC and c-Myc may play an important role in the progression of NSCLC.

关 键 词：肺肿瘤 APC C-MYC 预后 免疫组化 组织芯片 

分 类 号：R734.2[医药卫生—肿瘤]