羟基葫芦[6]脲与孟加拉红包结作用的荧光光谱研究  

Fluorometric study on the inclusion interaction of perhydroxycucurbit[6]uril with tetrachlorofluorescein

作　　者：从艳霞[1] 胡志雄[2] 张维农[1] 张寒俊[2] 王展[2] 

机构地区：[1]武汉工业学院食品科学与工程学院,武汉430023 [2]武汉工业学院化学与环境工程系院,武汉430023

出　　处：《分析试验室》2010年第10期16-20,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20575075);国家教育部基金(206099);武汉工业学院研究生教育创新计划(08CX025)项目资助

摘　　要：采用荧光光谱法研究了羟基葫芦[6]脲(HOCB[6])对孟加拉红(TSS)的包结作用,考察了HOCB[6]浓度、缓冲液pH、温度、包结时间、有机溶剂等因素对包结作用的影响,结果表明,体系的荧光强度随着HOCB[6]浓度的升高而增强,呈现显著荧光增敏现象,同时荧光峰位有一定蓝移,Hildebrand-Benesi法计算结果显示HOCB[6]与TSS形成了1∶1的包结配合物,包结反应的热力学参数表明该包结过程为自发放热过程,这可能是主客体分子之间的疏水作用与离子偶极作用所引起的。The inclusion interaction of perhydroxycucurbit[6]uril(HOCB[6]) with tetrachloro fluorescein(TSS) was studied by fluorometric method.The effect of some factors,such as concentration of HOCB[6],pH,temperature,inclusion time and common organic solvents on the fluorescence intensity and complex stability were investigated.The results indicate the emission peak of TSS appears a little blue shift and there is an obvious fluorescence enhancement along with the introduction of HOCB [6],the fluorescence intensity of the complex linear increased with the concentration of HOCB [6].The results obtained from the method Hildebrand-Benesi prove that the 1∶1 type inclusion complex is formed between HOCB [6] and TSS,and the thermodynamic parameters of inclusion reaction indicate that the inclusion process is a spontaneous exothermic process,which is mainly caused by the hydrophobic interaction and ion-dipole interaction between the host and guest molecules.

关 键 词：羟基葫芦[6]脲 脲 孟加拉红 荧光光谱法 包结作用 

分 类 号：O657.3[理学—分析化学]