盐度对细菌菌株降解苯酚的影响  

Influence on Phenol Degradation by Bacterial Strains under Different Salinity Conditions

作　　者：温洪宇[1,2] 杨柳[2] 韩宝平[1,2] 平文娟[2] 陈艳红[2] 马小琛[2] 

机构地区：[1]中国矿业大学环境与测绘学院,江苏徐州221008 [2]徐州师范大学生命科学学院,江苏徐州221116

出　　处：《环境科学与技术》2010年第11期33-38,共6页Environmental Science & Technology

基　　金：江苏省自然科学基金(BK2008121);江苏省高校自然科学基础研究项目(06KJD180180);江苏省遗传学重点学科建设项目资助(SYC0709);徐州师范大学培育课题(06DYL03);徐州师范大学自然科学基金项目(07XLB11)

摘　　要：从处理苯酚废水活性污泥中筛选分离到四株苯酚降解优势细菌菌株JHCFS1,JHCFS2,JHCFS3与JHCFS4,通过四株细菌在不同盐度条件下的苯酚降解率表明,随着盐浓度的逐渐升高,抑制作用逐渐增大,当NaCl,KCl浓度为4%时,四株细菌降解苯酚均受到显著抑制。苯酚浓度为1000mg/L,NaCl浓度为3%时,JHCFS2的降解率最高为83%,JHCFS4的降解率最低为47.20%;KCl浓度为3%时,JHCFS2的降解率最高为99%,JHCFS4的降解率最低为48%,表明四株菌在盐浓度(NaCl,KCl)低于3%的条件下可正常降解苯酚。通过对四株菌的16S rRNA基因克隆与序列分析,在NCBI进行BLAST获得同源性序列,利用Clustalx1.8软件和MEGA4.0软件进行同源性比较和系统发育学分析,结合生理生化特性将菌株JHCFS1,JHCFS2,JHCFS3与JHCFS4(GenBank收录号:FJ455076,FJ455077,FJ458437与FJ458438)分别归为Bacillus simplex,Bacillus cereus,Bacillus pumilus与Bacillus cibi,为降解苯酚提供了微生物物种资源。Four phenol degradation bacterial strains named JHCFS1,JHCFS2,JHCFS3 and JHCFS4 were isolated from activated sludge in phenol wastewater treatment system.Phenol degradation efficiency of 4 bacterial strains were studied under different salinity conditions,results indicated that degradation efficiencies of the 4 bacterial phenol decreased with increasing salinity and degradation efficiency was the lowest when NaCl and KCl concentration was 4% respectively.When the phenol concentration was 1,000mg/L,NaCl conc...

关 键 词：苯酚 盐度 16SrRNA 发育树 基因 

分 类 号：X172[环境科学与工程—环境科学]