小胶质细胞在SOD1-G93A转基因小鼠腰髓中的变化  

Change of microglia in the lumbar spinal cord of SOD1-G93A transgenic mice

作　　者：尹云霞[1] 张坤西[1] 郭艳苏[1] 李春岩[1] 李忠尧[1] 黄晶[1] 王倩[1] 吴红然[1] 

机构地区：[1]河北医科大学第二医院神经内科,石家庄050000

出　　处：《脑与神经疾病杂志》2010年第4期265-268,共4页Journal of Brain and Nervous Diseases

基　　金：国家自然科学基金项目(30900460;30870882)

摘　　要：目的观察小胶质细胞在SOD1-G93A转基因小鼠不同时期腰髓中的变化,探讨小胶质细胞活化与肌萎缩侧索硬化(ALS)疾病进展的关系。方法以国际公认的SOD1-G93A转基因小鼠,应用免疫组化、激光共聚焦显微镜及Westernblot方法 ,分别观察SOD1-G93A转基因小鼠症状前期、症状期、终末期及其同窝对照腰髓小胶质细胞形态数量及特异性标记物表达的变化情况。结果 SOD1-G93A转基因小鼠腰髓在症状前期(60天)已出现小胶质细胞数量增多及特异性标记物CD11b表达升高,随病程进展,症状期小胶质细胞增多、活化显著,终末期达高峰。结论随SOD1-G93A转基因小鼠病程进展小胶质细胞增生明显,小胶质细胞的活化可能参与ALS运动神经元损伤。Objective To observe the change of microglia in the lumbar spinal cord of SOD1-G93A transgenic mice at different stages and discuss the relationship between microglial activation and disease progression of amyotrophic lateral sclerosis(ALS).Methods The well-known mouse model of SOD1-G93A transgenic mice was used,morphology and number of microglia and expression of specific marker in the lumbar spinal cord were analyzed at presymptomatic,symptomatic and end stages of SOD1-G93A transgenic mice using immunohistochemistry,confocal microscopy and Western blot.Results Increased number and specific CD11b expression of microglia appeared at presymptomatic stage(60 days of age)in the lumbar spinal cord of SOD1-G93A transgenic mice,and became obvious at symptomatic stage and reached the maximum at end stage.Conclusion Microglia proliferates markedly with the disease progression of SOD1-G93A transgenic mice.Activation of microglia may contribute to motor neuron injury of ALS.

关 键 词：肌萎缩侧索硬化 脊髓 小胶质细胞 CD11B Iba1 转基因小鼠 

分 类 号：R744.8[医药卫生—神经病学与精神病学]