尿多酸肽对人肺腺癌A549细胞的作用及人肺腺癌A549细胞株E-cadherin基因甲基化的研究  

The Impact of CDA-Ⅱ on A549 Cells and Detection of Methylation of E-cadherin Gene in Lung Cancer Cell Line A549

作　　者：王红兵[1] 王亚丽[2] 刘德生[1] 徐海洋[1] 裴冬生[3] 祖茂衡[1] 

机构地区：[1]徐州医学院附属医院,221002 [2]徐州医学院,在读研究生221002 [3]徐州医学院肿瘤生物治疗实验室,221002

出　　处：《实用癌症杂志》2010年第5期445-446,452,共3页The Practical Journal of Cancer

摘　　要：目的探讨尿多酸肽(CDA-Ⅱ)对人肺腺癌A549细胞的作用及检测人肺腺癌A549细胞株E-cadherin基因甲基化的状况,寻找肺癌治疗的新靶点。方法采用MTT法检测不同浓度尿多酸肽作用下A549细胞的存活率。甲基化特异性PCR(MSP)检测人肺腺癌A549细胞株E-cadherin基因甲基化的状况。结果尿多酸肽显著抑制A549细胞生长,随着剂量的增加其抑制作用增强。A549细胞株存在E-cadherin基因异常甲基化,呈部分甲基化。结论尿多酸肽具有抑制A549细胞生长的作用,A549细胞株存在E-cadherin基因的异常甲基化。Objective To study the anti-tumor effect of CDA-Ⅱ on A549 cells and to detect the methylation pattern of E-cadherin gene inr cell line A549.Methods The growth inhibition of A549 cell induced by CDA-Ⅱ was detected by MTT.Methylation specific PCR (MSP) was utilized to examine methylation status of E-cadherin gene in lung cancer cell line A549.Results CDA-Ⅱ showed significant inhibiting effect on A549 cells,exhibiting a dose-dependent relationship.E-cadherin methylation was positive in lung cancer cell line A549.Conclusion CDA-Ⅱ can inhibit the growth of A549 cells and hypermethylation of E-cadherin gene exists in lung cancer cell line A549.

关 键 词：尿多酸肽 A549细胞 甲基化特异性PCR 上皮钙黏附素 

分 类 号：R734.2[医药卫生—肿瘤]