In Vitro Interaction of Nicotine and Hemoglobin under Liver Cell Metabolizing Condition  

作　　者：Yan WANG Hong Fang SUN Hai Fang WANG Yuan Fang LIU 

机构地区：[1]Department of Technical Physics,Peking University,Beijing 100871

出　　处：《Chinese Chemical Letters》2001年第5期449-452,共4页中国化学快报(英文版)

基　　金：We thank the financial support of the National Natural Science Foundation of China(Grant.No.19935020);Research Fund for Doctoral Program of Higher Education.

摘　　要：The in vitro interaction of nicotine and hemoglobin (Hb) in a metabolizing System was studied by spectroscopy assays. Visible spectra showed two isobestics, and fluorescence spectra showed static quenching with increasing of nicotine dose. Meanwhile, the CD spectra intensity reduced, showing the conformation of Hb varied markedly through the interaction. All these results suggested that the interaction of nicotine or its metabolites and Hb might do harm to physicological function of Hb.The in vitro interaction of nicotine and hemoglobin (Hb) in a metabolizing System was studied by spectroscopy assays. Visible spectra showed two isobestics, and fluorescence spectra showed static quenching with increasing of nicotine dose. Meanwhile, the CD spectra intensity reduced, showing the conformation of Hb varied markedly through the interaction. All these results suggested that the interaction of nicotine or its metabolites and Hb might do harm to physicological function of Hb.

关 键 词：NICOTINE HEMOGLOBIN visible absorption FLUORESCENCE CD 

分 类 号：Q26[生物学—细胞生物学]