Studies on Antineoplastic Constituents from Marine Bryozoan Bugula neritina Inhabiting South China Sea (Ⅲ): Isolation and Structural Elucidation of Bryostatins 10,11 and 18  

作　　者：林厚文 易杨华 姚新生 吴厚铭 

机构地区：[1]School of Pharmacy, Second Military Medical University, Shanghai 200433 [2]Department of Natural Products, Shenyang Pharmaceutical University, Shenyang 110015, China [3]Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, Chinaix active compounds are isolated from the marine bryozoan Bugula neritina, inhabiting the Nanwan Bay in the South China Sea, using the bioassay-guided method with a combination of extraction and partitionation with suitable solvents as well as multiple column chromatographies ( Sephadex LH-20, ODS and preparative HPLC ). Their structures are identified as known bryostatins- bryostatins 4, 5, 6, 10, 11 and 18 through intensive analysis of the data of high resolution 2D NMR (600 MHz, DQF-COSY, TOCSY, HMQC and ROESY) and ESI-MS. Among them, bryostatins 10, 11 and 18 are for the first time obtained from this bryozoan in the South China Sea and they show significant antineoplastic activities in vitro.

出　　处：《Marine Science Bulletin》2001年第1期83-90,共8页海洋通报（英文版）

基　　金：the National Natural Science Foundation of China(NNSFC,29672050).

摘　　要：Six active compounds are isolated from the marine bryozoan Bugula neritina,inhabiting the Nanwan Bay in the South China Sea, using the bioassay-guided method with a combination of extraction and partitionation with suitable solvents as well as multiple column chromatographies ( Sephadex LH-20, ODS and preparative HPLC).Their structures are identified as known bryostatins-bryostatins 4, 5, 6, 10, 11 and 18 through intensive analysis of the data of high resolution 2D NMR (600 MHz, DQF-COSY,TOCSY, HMQC and ROESY) and ESI-MS. Among them, bryostatins 10, 11 and 18 are for the first time obtained from this bryozoan in the South China Sea and they show significant antineoplastic activities in vitro.Six active compounds are isolated from the marine bryozoan Bugula neritina, inhabiting the Nanwan Bay in the South China Sea, using the bioassay-guided method with a combination of extraction and partitionation with suitable solvents as well as multiple column chromatographies ( Sephadex LH-20, ODS and preparative HPLC ). Their structures are identified as known bryostatins- bryostatins 4, 5, 6, 10, 11 and 18 through intensive analysis of the data of high resolution 2D NMR (600 MHz, DQF-COSY, TOCSY, HMQC and ROESY) and ESI-MS. Among them, bryostatins 10, 11 and 18 are for the first time obtained from this bryozoan in the South China Sea and they show significant antineoplastic activities in vitro.

关 键 词：Bugula neritina  ANTINEOPLASTIC activities  bryostatins 

分 类 号：R284[医药卫生—中药学]