端粒酶逆转录酶蛋白及mRNA在胃癌中的表达及相关性研究  

Study on the correlation and expression of hTERT protein and hTERTmRNA in gastric cancer.

作　　者：张赟[1] 伍冬梅[1] 李春鸣[2] 

机构地区：[1]贵州省人民医院病理科,贵阳550002 [2]遵义医学院病理教研室

出　　处：《贵州医药》2011年第3期211-214,共4页Guizhou Medical Journal

基　　金：贵州省优秀青年科技人才培养计划基金资助;项目编号[黔科合人字(20005)0512]

摘　　要：目的通过对胃癌组织中hTERT蛋白及hTERTmRNA表达的研究,探讨hTERT与胃癌临床病理特征的关系和意义。方法采用免疫组化法及原位杂交法检测10例正常胃组织和51例胃癌组织中hTERT蛋白及hTERTmRNA表达情况。结果胃癌中hTERT蛋白及hTERTmRNA阳性表达率显著高于正常胃粘膜;胃癌中hTERT蛋白表达阳性率与患者发生部位及患者的年龄显著相关。hTERT蛋白阳性胃癌标本中hTERTmRNA阳性为74.36%;hTERT蛋白阴性的胃癌标本中,hTERTmRNA阳性为0%。结论 hTERT与胃癌的发生有关,且在胃癌中呈高表达。hTERT蛋白与hTERTmRNA在胃癌中表达量呈正相关,其阳性表达可能是胃癌的标志之一。Objective To search for the clinicopathologic characteristics and the significance of the relationship in gastric cancer through studied in gastric expression of hTERT protein and hERTmRNA expression.Methods 10 cases of normal gastric tissues and 51 cases of gastric carcinoma the expression of hTERT protein and hTERTmRNA were detected by immunohistochemistry and in situ hybridization.Results The hTERT protein and hTERTmRNA gastric expression were significantly higher than in normal gastric mucosa.There was ...

关 键 词：胃癌 端粒酶逆转录酶 HTERTMRNA 免疫组织化学 原位杂交 

分 类 号：R735.2[医药卫生—肿瘤]