马铃薯Desiree遗传转化体系的优化及转基因植株的获得  

Optimization of Genetic Transformation System for Potato Variety 'Desiree' and Obtainment of Transgenic Lines

作　　者：辛翠花[1,2] 郭江波[2] 黄三文[1] 屈冬玉[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081 [2]内蒙古科技大学数理与生物工程学院,内蒙古包头014010

出　　处：《中国蔬菜》2011年第6期15-21,共7页China Vegetables

基　　金：国家自然科学基金项目(30671319);中荷园艺作物基因组分析联合实验室运行项目(1251601001);国家重点基础研究发展计划项目(2006cb101907);中荷战略联盟项目(2004CB720405)

摘　　要：为优化马铃薯品种Desiree的遗传转化体系并获得转基因马铃薯,以Desiree叶片和茎段为外植体,分别比较了不同转化条件(预培养时间、菌液浓度、侵染时间及激素配比)对遗传转化效率的影响。结果表明:以茎段为外植体,预培养2d后以OD600=0.5的菌液侵染10min,在加有1.0mg.L-1反式玉米素(ZT-t)的MS20选择培养基上的遗传转化率最高,其愈伤诱导率可达80%,芽分化率可达70%。通过该转化体系将抗晚疫病基因R1导入马铃薯,获得了具有卡那霉素抗性的转化植株。经PCR检测和抗病接种鉴定,外源基因已经导入马铃薯基因组中,获得的转基因马铃薯具有很强的抗晚疫病能力,且转基因马铃薯植株和块茎的重要农艺性状没有发生改变。The aim of this work was to optimize the transformation system for potato(Solanum tuberosum L.)variety Desiree and to obtain transgenic potato.Explants derived from potato leaves and stems were used as receptor materials to compare transformation efficiency with different time of pre-culture,Agrobacterium tumefaciens content,duration of infection and the proportion of hormones.The results showed that stem was most suitable for the induction of callus and shoots.Stem explants were pre-cultured for 2 days bef...

关 键 词：马铃薯 遗传转化 外植体 

分 类 号：S532[农业科学—作物学]