HMME-PDT对人舌鳞癌Tca8113细胞的作用及影响因素初步研究  

Preliminary Study of the Effect of HMME-mediated PDT on Human Tongue Squamous Cell Carcinoma Cell Line Tca8113 and the Possible Factors Involved

作　　者：赖兴强 刘建中 李晓原[2] 胡江[2] 吴俊超[2] 唐琳[2] 

机构地区：[1]中山大学中山医学院生物学教研室,广东广州510080 [2]中山大学中山医学院生物医学工程系,广东广州510080

出　　处：《中国医学物理学杂志》2011年第2期2563-2566,2577,共5页Chinese Journal of Medical Physics

摘　　要：目的:初步探讨血卟啉单甲醚(HMME)介导的光动力疗法(HMME-PDT)对人舌鳞癌Tca8113细胞的作用及可能的影响因素。方法:以HMME作为光敏剂、发光二极管(LED)为光源的光动力疗法作用于Tca8113细胞。采用MTT法分别检测光敏剂孵育时间、光敏剂浓度、光剂量及光功率密度对Tca8113细胞抑制率的影响。HE染色观察细胞形态学改变,Hoechst 33342荧光染色观察细胞核凋亡情况。结果:细胞抑制率随光敏剂孵育时间延长而增大,呈时间依赖效应;并且随光敏剂浓度和光剂量增加而增大,呈浓度-光剂量依赖效应;在相同光剂量下,抑制率随光功率密度增加而增大,在高剂量时更明显。HE染色显示,与对照组相比,PDT处理组细胞密度明显降低,死细胞明显增多;Hoechst 33342荧光染色可见核染色质浓集、核固缩、核碎裂,出现凋亡小体,呈典型凋亡形态改变。结论:HMME-PDT能有效杀伤Tca8113细胞,光敏剂孵育时间、光敏剂浓度、光剂量及光功率密度以均是影响PDT疗效的重要因素,HMME-PDT主要通过凋亡方式诱导细胞死亡。Objective: To investigate the effect of HMME-mediated PDT(HMME-PDT) on human tongue squamous cell carcinoma cell line Tca8113 and the possible factors involved.Methods: The PDT treatment on Tca8113 cells was performed in vitro using HMME as photosensitizer and light-emitting diode(LED) as light source.MTT assay was used to evaluate the incubation time and concentration of HMME,the dosages and power densities of light on the effect of the inhibition rate of Tca8113 cells.The morphological changes of cells we...

关 键 词：光动力疗法 血卟啉单甲醚 舌鳞状细胞癌 TCA8113细胞 凋亡 

分 类 号：R739.8[医药卫生—肿瘤]