组织因子在纤维肉瘤细胞血管外游走中的作用  

Effect of tissue factor in extravascular migration of fibrosarcoma cells

作　　者：韩春山[1] 辛华[1] 韩振国[1] 高忠礼[2] 

机构地区：[1]吉林大学中日联谊医院胸外科,吉林长春130033 [2]吉林大学中日联谊医院骨科,吉林长春130033

出　　处：《浙江大学学报（医学版）》2011年第2期184-188,共5页Journal of Zhejiang University(Medical Sciences)

基　　金：吉林省杰出青年科技支持计划(20070140)资助项目

摘　　要：目的:评价组织因子对纤维肉瘤细胞血管外游走的影响,明确组织因子与纤维肉瘤细胞血行转移的相关性。方法:应用流式细胞仪检测组织因子在纤维肉瘤细胞(HT1080)的表达情况;在体外模拟肿瘤细胞血管外游走过程,构建充分融合的单层血管内皮细胞及血管外基质,定量评价HT1080向血管外游走的状况。结果:应用流式细胞仪检测出组织因子在HT1080上有强表达;HT1080可以穿过单层血管内皮细胞游走至血管外基质,具有时间依赖性;抗组织因子抗体能够抑制HT1080的血管外游走,并且高浓度的抗TF抗体(20 mg/L)较低浓度的抗TF抗体(5 mg/L)的抑制作用更为明显,差异有显著意义(P<0.05),具有浓度依赖性。结论:组织因子能够促进纤维肉瘤细胞的血管外游走,从而加速纤维肉瘤细胞的血行转移。Objective:To evaluate the effect of tissue factor(TF) in extravascular migration of fibrosarcoma cells and hematogenous metastasis. Methods:The expression of tissue factor in fibrosarcoma HT1080 cells was analyzed by flow cytometry.The extravascular migration of fibrosarcoma cells was observed in a constructed monolayer vascular endothelial cells and extra-cellular matrix model. Results:Tissue factor was highly expressed in HT1080 cells.HT1080 migrated and passed through the monolayer vascular endothelial cells to the collagen gel in a time-dependent manner.Anti-TF antibody inhibited extravascular migration of fibrosarcoma cells and the inhibition was concentration-dependent(P<0.05). Conclusion:Tissue factor may enhance hematogenous metastasis through extravascular migration of fibrosarcoma cells.

关 键 词：纤维肉瘤/病理学 血管/代谢 肿瘤转移 组织因子 纤维肉瘤细胞 血管外游走 

分 类 号：R730.2[医药卫生—肿瘤]