白藜芦醇在诱导人胃癌HGC27细胞凋亡中的机制研究  

Mechanism of apoptosis induced by Resveratrol in human gastric cancer HGC27 cells

作　　者：杨宏梅[1] 邢影[1] 郑美珍[1] 

机构地区：[1]辽宁省肿瘤医院内科,沈阳110042

出　　处：《上海交通大学学报（医学版）》2011年第2期187-190,共4页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的探讨白藜芦醇(Res)干预人胃癌HGC27细胞后,PTEN与p-Akt信号通路中,VEGF、MMP-9表达差异并探讨其相互关系。方法应用体外HGC27细胞培养技术,采用CCK-8实验测定Res对HGC27细胞生长抑制率的影响;采用流式细胞术分析细胞周期;采用Western blotting检测Res对HGC27细胞中凋亡相关基因PTEN和p-Akt的影响;采用ELISA法检测Res干预前后HGC27细胞的VEGF和MMP-9蛋白分泌水平的变化。结果 Res对HGC27细胞的生长抑制率存在浓度和时间依赖性(P<0.05);Res干预HGC27后48 h,细胞周期分析显示DNA合成期(S期)细胞比例明显减少,并呈现剂量相关性(r=-0.930,P=0.007);PTEN相对表达量与Res干预浓度呈正相关(r=0.951,P=0.04);p-Akt、VEGF和MMP-9相对表达量均与Res干预浓度呈负相关(r=-0.969,P=0.03;r=-0.894,P=0.02;r=-0.881,P=0.01)。结论 Res能够抑制人胃癌HGC27细胞的增殖,并且诱导其凋亡,其机制可能与上调PTEN的表达和抑制Akt的磷酸化有关,并通过调控VEGF和MMP-9的分泌实现HGC27的增殖抑制及凋亡。Objective To investigate the relationship between the expression of VEGF and MMP-9 in PTEN and p-Akt signaling pathway of resveratrol affecting gastric cancer HGC27 cells. Methods The effects of resveratrol on growth inhibition rate and cell cycle of HGC27 cells were examined by CCK-8 test and flow cytometry,respectively.The effects of resveratrol on apoptosis-related PTEN and p-Akt gene in HGC27 cells were detected by Western blotting,and the contents of VEGF and MMP-9 protein in HGC27 cells before and after resveratrol treatment were determined by ELISA. Results The growth inhibition rate of HGC27 cells by resveratrol treatment was concentration-and time-dependent(P<0.05).Forty-eight hours after resveratrol treatment,cell cycle analysis indicated that the percent of HGC27 cells with DNA synthesis(S phase) significantly decreased,which exhibited a negative correlation with dosage(r=-0.930,P=0.007).There was a positive relationship between the relative expression of PTEN and Res concentration(r=0.951,P=0.04),while there was a negative relationship between the relative expression of p-Akt,VEGF and MMP-9 and Res concentration(r=-0.969,P=0.03;r=-0.894,P=0.02;r=-0.881,P=0.01). Conclusion Resveratrol could inhibit the proliferation and induce apoptosis of human gastric cancer HGC27 cells,which may be related to the expression up-regulation of PTEN and phosphorylation inhibition of Akt.Resveratrol could also regulate the expression of VEGF and MMP-9 to inhibit the proliferation and apoptosis of HGC27 cells.

关 键 词：白藜芦醇 HGC27细胞 细胞凋亡 PTEN P-AKT 

分 类 号：R735.2[医药卫生—肿瘤]