异位子宫内膜17β-羟基类固醇脱氢酶2缺乏的分子机制  

Mechanism of 17β-hydroxysteroid dehydrogenase-2 deficiency in ectopic endometrium

作　　者：张萍[1] 李笛悠[1] 惠宁[2] 

机构地区：[1]上海交通大学医学院附属新华医院妇科,上海200092 [2]第二军医大学附属长海医院妇产科,上海200433

出　　处：《上海交通大学学报（医学版）》2011年第4期510-512,527,共4页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：正常子宫内膜凋亡受雌激素的影响,17β-羟基类固醇脱氢酶2(17β-HSD2)在调控雌激素生物活性中起主要作用,其量和活性依赖孕激素的调控,孕激素通过与孕激素受体结合起作用。在子宫内膜异位症患者的异位内膜中,17β-HSD2表达下降。文章综述17β-HSD2对雌激素活性的调控及对子宫内膜生长的调节,以及17β-HSD2的表达与孕激素及其相应受体结构、基因、亚型、功能的相关性,并探讨17β-HSD2在子宫内膜异位症的发生、发展、治疗、预后中的作用。Objective To investigate the mechanism of changes of isoforms of local progesterone receptors(PRs) and 17β-hydroxysteroid dehydrogenase 2(17β-HSD2) deficiency in insensitiveness of ectopic endometrium(EMT) to progestin. MethodsEctopic endometrium samples of 36 cases of EMT(EMT ectopic endometrium group) and 16 samples of eutopic endometrium(EMT eutopic endometrium group) were selected,the expression of PR-(A+B),PR-A,PR-B and 17β-HSD2 mRNA was detected by Real-Time PCR,and the expression of PR-(A+B),PR-B and 17β-HSD2 protein was determined by immunohistochemistry. ResultsIn EMT eutopic endometrium group,the expression of PR-(A+B),PR-B and PR-A mRNA in proliferative phase was significantly higher than that in secretory phase(P<0.05).PRs were expressed in both endometrial epithelial and stromal cells in proliferative phase,while those were mainly expressed in stromal cells in secretory phase.PR-A gained the advantage between PR-A and PR-B,while the rate of PR-A/PR-B was relatively stable during the whole menstrual cycle.The expression of PR-B in endometrial stromal cells was positively correlated with that of 17β-HSD2 in epithelial cells.In EMT ectopic endometrium group,the expression of PR-A was continuously low in the whole menstrual cycle,while there was no significant difference between the expression of PR-A in ectopic endometrium and that in eutopic endometrium in secretory phase(P>0.05).The expression of PR-B in ectopic endometrium was significantly lower than that in eutopic endometrium in the whole menstrual cycle,and the cyclical variations of PR-A and PR-B disappeared in ectopic endometrium.Compared with EMT eutopic endometrium group,the expression of PR-B in EMT ectopic endometrium group was significantly lower,and the rate of PR-A/PR-B was significantly higher(P=0.001).In EMT ectopic endometrium group,PR-B was significantly lower in stromal cells,17β-HSD2 also decreased significantly,and the cyclical variation of 17β-HSD2 disappeared. ConclusionThe changes of isoforms of PRs in ectopic endometriu

关 键 词：子宫内膜异位症 17β-羟基类固醇脱氢酶2 雌激素 孕激素 孕激素受体 维甲酸 代谢 

分 类 号：R[医药卫生]