Twist在宫颈鳞癌中的表达及其与临床生物学行为的关系  

Expression of Twist in cervical cancer and its relationship with clinical biological behaviors

作　　者：鲍伟[1] 刘小奕[1] 许颖[1] 丁李[1] 吴佳皓[1] 李柱南[1] 吴丹[1] 

机构地区：[1]上海交通大学医学院附属国际和平妇幼保健院,上海200030

出　　处：《上海交通大学学报（医学版）》2011年第5期547-550,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市科委基金(09ZR1435000)~~

摘　　要：目的探讨转录因子Twist在人宫颈鳞癌、宫颈上皮内瘤样变(CIN)和正常宫颈上皮组织中的表达及临床病理意义。方法收集宫颈鳞癌标本(宫颈鳞癌组,n=61)、CINⅠ期标本(CINⅠ期组,n=22)、CINⅡ～Ⅲ期标本(CINⅡ～Ⅲ期组,n=44)以及正常宫颈组织标本(正常对照组,n=22),采用免疫组织化学方法检测宫颈组织中Twist蛋白的表达情况,并分析其与宫颈鳞癌临床生物学行为的关系。结果正常对照组、CINⅠ期组、CINⅡ～Ⅲ期组和宫颈鳞癌组的Twist蛋白阳性率呈逐渐上升趋势,分别为0、40.90%、68.18%和70.49%;CINⅠ期组、CINⅡ～Ⅲ期组和宫颈鳞癌组的Twist蛋白阳性率与正常对照组比较,差异均具有统计学意义(P<0.01);在CINⅠ期组、CINⅡ～Ⅲ期组及宫颈鳞癌组间比较,Twist蛋白阳性率差异无统计学意义(P>0.05)。结合临床病理分析,在宫颈鳞癌组中,不同年龄、FIGO临床分期及有无淋巴血管间隙浸润的患者间比较,Twist蛋白阳性率差异均无统计学意义(P>0.05);Twist蛋白在低分化和未分化肿瘤中的阳性率(94.44%)明显高于高、中分化肿瘤(60.47%),差异有统计学意义(P<0.05);有淋巴结转移者Twist蛋白阳性率(93.75%)较无淋巴结转移者(62.22%)明显升高(P<0.05)。结论 Twist蛋白在宫颈鳞癌中高表达,在判断肿瘤分化程度及转移潜能方面有辅助诊断价值。Objective To investigate the expression of transcription factor Twist in human cervical cancer(squamous cell carcinoma),cervical intraepithelial neoplasia(CIN) and normal cervical tissues,and explore its clinicopathological significance. Methods Samples of cervical cancer(cervical cancer group,n=61),CINⅠ(CINⅠgroup,n=22),CINⅡ-Ⅲ(CINⅡ-Ⅲ group,n=44) and normal cervical tissues(normal control group,n=22) were collected,the expression of Twist protein in cervical tissues was detected by immunohistochemistry,and its relationship with clinical biological behaviors of cervical cancer was analysed. Results The expression rates of Twist protein in normal control group,CIN Ⅰ group,CINⅡ-Ⅲ group and cervical cancer group were 0,40.90%,68.18% and 70.49%,respectively.The expression rates of Twist protein in cervical cancer group,CINⅠ group and CINⅡ-Ⅲ group were significantly higher than that in normal control group(P<0.01).However,there was no significant difference in the expression rates of Twist protein among cervical cancer group,CIN Ⅰ group and CINⅡ-Ⅲ group(P>0.05).There was no significant difference in the expression rates of Twist protein among patients with different ages,FIGO clinical stages and lymphovascular space invasion status in cervical cancer group(P>0.05).The expression rate of Twist protein in poorly-differentiated and undifferentiated tumors(94.44%) was significantly higher than that in well-differentiated and medium-differentiated tumors(60.47%)(P<0.05),and the expression rate of Twist protein in patients with lymph node metastasis(93.75%) was significantly higher than that in those without lymph node metastasis(62.22%)(P<0.05). Conclusion There is high expression of Twist protein in cervical cancer,which may be helpful in the evaluation of differentiation and metastasis of cancer.

关 键 词：TWIST基因 转录因子 宫颈鳞癌 宫颈上皮内瘤样变 免疫组织化学 

分 类 号：R737.33[医药卫生—肿瘤]