急性运动轴索型神经病患者血清对大鼠脊髓运动神经元凋亡相关蛋白表达的影响  

Effects of serum of patients with acute motor axonal neuropathy on expression of apoptosis-related proteins in rat spinal motor neuron

作　　者：彭立威[1] 杨国锋[1] 纪建国[2] 魏晓姗[1] 李培[1] 赵景茹[1] 张锐利[1] 冯晓[1] 

机构地区：[1]河北医科大学第二医院神经科,石家庄050000 [2]北京大学生命科学学院蛋白质工程国家重点实验室,北京100871

出　　处：《上海交通大学学报（医学版）》2011年第5期580-583,591,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(30500164);河北省自然科学基金(C2007000848)~~

摘　　要：目的采用蛋白质组学技术研究急性运动轴索型神经病(AMAN)患者血清对大鼠脊髓运动神经元凋亡相关蛋白表达的影响。方法分别以16例AMAN患者和16名健康献血者的血清对原代培养大鼠脊髓运动神经元进行干预,24 h后行双向凝胶电泳,MALDI-TOF/TOF串联质谱鉴定神经元发生变化的蛋白,表达水平上升或下降超过30%的点判定为差异蛋白质点。结果 AMAN患者血清干预与健康献血者血清干预的大鼠脊髓运动神经元比较,有21种差异表达蛋白,其中内质网蛋白29、不均一核糖核蛋白H、蛋白酶体α亚基1、3-磷酸甘油醛脱氢酶、电压门控阴离子选择性通道蛋白1为凋亡相关蛋白。结论在AMAN患者血清作用下,大鼠脊髓运动神经元的细胞凋亡相关蛋白表达发生了变化。Objective To investigate the effects of serum of patients with acute motor axonal neuropathy(AMAN) on expression of apoptosis-related proteins in rat spinal motor neuron with proteomics. Methods Serum samples of 16 patients with AMAN and 16 healthy people were obtained,and two-dimensional gel electrophoresis was performed 24 h after primary culture of rat spinal motor neuron.The proteins of interest were identified by MALDI-TOF/TOF tandem mass spectrometry,and differential protein spots were defined as those with changes of expression >30%. Results There were 21 differentially expressed proteins,among which heterogeneous nuclear ribonucleoprotein H,proteasome subunit alpha type 1,endoplasmic reticulum protein 29,glyceraldehyde-3-phosphate dehydrogenase and voltage-dependent anion-selective channel protein 1 were apoptosis-related proteins. Conclusion Serum of patients with AMAN may lead to changes of expression of apoptosis-related proteins in rat spinal motor neuron.

关 键 词：急性运动轴索型神经病 蛋白质组学 血清 凋亡相关蛋白 

分 类 号：R744[医药卫生—神经病学与精神病学]