前体mRNA可变剪接调节因子NSSR1在大肠癌中的表达  

Expression of neural salient serine-/arginine-rich protein 1(NSSR1) in colorectal cancer

作　　者：张薇[1] 沈权[1] 沈嘉希[1] 

机构地区：[1]浙江大学城市学院医学与生命科学学院,浙江杭州310015

出　　处：《浙江大学学报（医学版）》2011年第5期540-544,共5页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省教育厅科研资助项目(Y201017203)

摘　　要：目的:研究神经系统显著表达的前体mRNA可变剪接调节因子NSSR1在大肠癌中的表达。方法:应用RT-PCR、Western blot以及免疫组织化学染色方法观测小鼠肠道等组织和人类大肠癌中NSSR1的mRNA和蛋白的表达以及分布。结果:NSSR1的mRNA在小鼠的大脑、小脑、心脏、肝脏、大肠以及肺等组织中均有表达,而蛋白只在神经系统表达,在肠道中无表达。在人体正常肠道组织中,NSSR1只在肠黏膜上皮细胞中表达;而在大肠癌细胞中,NSSR1存在显著表达且主要分布在细胞核中。结论:NSSR1在大肠癌中高表达,这可能与NSSR1在大肠癌发生发展中的生物学功能有关。Objective: To investigate the expression of neural salient serine/arginine-rich protein 1(NSSR1) in colorectal cancer. Methods: RT-PCR,Western blot and immunohistochemical staining were used to detect the expression of NSSR1 mRNA and peotein in different mouse tissues and human colorectal cancer,respectively. Results: NSSR1 mRNA was expressed in mouse cerebrum,cerebellum,heart,liver,intestine,kidney and lung tissue,but NSSR1 protein was only expressed in neural tissues.In normal human intestinal mucosa,NSSR1 was expressed in the colorectal epithelial cells.In colorectal cancer,NSSR1 was highly expressed in the nucleus of tumor cells. Conclusion: The extensive expression of NSSR1 in colorectal cancer cells may hint it`s roles in the biological function of colorectal cancer.

关 键 词：结直肠肿瘤 精氨酸/遗传学 丝氨酸/遗传学 RNA 信使 剪接体 免疫组织化学 基因表达 蛋白质类/遗传学 

分 类 号：R735.34[医药卫生—肿瘤]