外源过氧化物酶对辣椒果实抗氧化酶活性与品质的影响  

Effect of External Peroxidase on the Activities of Antioxidant Enzymes in Pepper

作　　者：杨静[1] 李小娟[1] 王丽[1] 穆春[1] 王林嵩[1] 徐扬[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《北方园艺》2011年第8期1-4,共4页Northern Horticulture

基　　金：国家自然科学基金资助项目(30570135);河南省生物化学与分子生物学重点学科资助项目

摘　　要：为了探讨外源萝卜过氧化物酶(RsPOD)对果蔬类作物影响的普遍性,将RsPOD定期喷洒辣椒植株,并测定辣椒果实的抗氧化酶(过氧化物酶、超氧化物歧化酶、过氧化氢酶)活性、可溶性蛋白含量、维生素C含量和叶绿素含量等生化指标以及POD同工酶谱。结果表明:Rs-POD不同程度地提高了这些生化指标在辣椒中的活性和含量,增强甚至提前了一些POD同工酶的表达。由此进一步证明外源RsPOD可以促进果蔬类作物的抗氧化能力和营养价值,具有一定的经济价值。In order to investigate further the effect of the external radish peroxidase(RsPOD) on the growth of vegetables,the different concentrations of RsPOD were sprayed periodically on the pepper.After then the activities of POD,superoxidase dismutase(SOD) and catalase(CAT),the contents of vitamin C,the soluble proteins,and the chlorophyll were determined.Besides,the patterns of peroxidase isozyme were measured by polyacrylamide gel electrophoresis.The results showed that,to some extent,the external RsPOD increased the activities of POD,SOD, and CAT and the contents of the soluble proteins and chlorophyll.Moreover,the external RsPOD enhanced,even advanced the expression of the peroxidase isozyme.Therefore,the external RsPOD can increase antioxidant ability and nutrient in vegetables.

关 键 词：过氧化物酶 过氧化氢酶 超氧化物歧化酶 可溶性蛋白 维生素C 叶绿素 

分 类 号：S6[农业科学—园艺学]