海洋生物活性药物BA-TPQ和FBA-TPQ对胃癌细胞的作用  

Mechanism and anticancer activity of makaluvamine analogues BA-TPQ and FBA-TPQ on gastric cancer cells

作　　者：徐哲[1] 张徐[1] 钱茜[1] 孙靖[1] 钱晖[1] 朱伟[1] 许文荣[1] 

机构地区：[1]江苏大学基础医学与医学技术学院,江苏镇江212013

出　　处：《江苏大学学报（医学版）》2011年第3期220-223,228,共5页Journal of Jiangsu University：Medicine Edition

基　　金：国家自然科学基金资助项目(30840053);江苏大学科技创新团队和拔尖人才项目(2008-018-02)

摘　　要：目的:观察两种海洋生物活性药物BA-TPQ和FBA-TPQ对人胃癌细胞株BGC-823的作用与机制。方法:采用四唑氮蓝(MTT)法测定不同浓度BA-TPQ和FBA-TPQ对BGC-823细胞的生长作用,应用流式细胞术检测BA-TPQ和FBA-TPQ对BGC-823细胞增殖和细胞周期的影响,应用蛋白质印迹法检测BA-TPQ和FBA-TPQ对BGC-823细胞信号通路的影响。结果:BA-TPQ和FBA-TPQ对BGC-823细胞有明显的体外增殖抑制作用,24 h的半数抑制浓度(IC50)分别为0.5986和0.6217μmol/L,且有明显的剂量依赖关系;BA-TPQ和FBA-TPQ作用BGC-823后S期细胞数增加,细胞出现明显的凋亡;BA-TPQ和FBA-TPQ体外通过下调磷酸化的细胞外信号调节激酶(P-ERK)和Bcl-2蛋白的表达发挥抗肿瘤作用。结论:BA-TPQ和FBA-TPQ对人胃癌细胞株BGC-823有生长抑制作用,其机制可能与阻滞细胞周期和诱导凋亡有关。Objective: To investigate the mechanism and anticancer effect of BA-TPQ and FBA-TPQ on the proliferation of gastric cancer cells(BGC-823) in vitro.Methods: Cell proliferation in different concentration was determined by Methyl thiazolyl tetrazolium(MTT) assay.The fluorescence flow cytometry(FCM) was applied to detect the apoptosis induction and alteration of cell cycle phase.The effect of BA-TPQ and FBA-TPQ on the signaling pathway of BGC-823 cells were detected by Western-blot.Results: After 24 h of treatment,the IC50 value were 0.5986,0.6217 μmol/L.BA-TPQ and FBA-TPQ inhibited the proliferation of gastric cancer BGC-823 cells in a concentration depended manner.FCM assay showed that the cells in S were increased after the treatment of two drugs.The Western-blot results showed that the expression of Bcl-2 and P-ERK protein were down regulated.Conclusion: BA-TPQ and FBA-TPQ can inhibit proliferation of human gastric cancer BGC-823 cells in vitro.The inhibition may be related to arrest cell cycle phase and apoptosis.

关 键 词：海洋生物活性药物 胃癌 胃癌细胞系 细胞凋亡 

分 类 号：R[医药卫生]