酸浆醇提物对食管癌EC-1.71细胞增殖的抑制作用  

Inhibition of proliferation on human esophageal cancer EC-1.71 cell by ethanol extracts of Physalis alkekengi Linn.

作　　者：孔静 邵世和[2] 陈浩宁 高小焕[1] 贾蓓蓓[2] 赵建中 李彬先[4] 

机构地区：[1]江苏大学生命科学研究院,江苏镇江212013 [2]江苏大学基础医学与医学技术学院,江苏镇江212013 [3]江苏大学附属医院骨科,江苏镇江212001 [4]北华大学附属医院检验科,吉林吉林132013

出　　处：《江苏大学学报（医学版）》2011年第3期233-236,共4页Journal of Jiangsu University：Medicine Edition

基　　金：江苏大学科技创新团队基金资助项目(2008-018-02);江苏大学高级人才启动基金资助项目

摘　　要：目的:研究酸浆醇提物(ethanol extracts of Physalis alkekengi Linn.,EEP)对食管癌EC-1.71细胞增殖的抑制作用,探讨其可能的作用机制。方法:EEP作用于EC-1.71细胞后,采用倒置显微镜观察细胞形态的改变;采用细胞计数法观察其对细胞增殖的抑制作用;通过流式细胞仪检测法分析EEP对EC-1.71细胞周期的影响。结果:EEP作用后,EC-1.71细胞出现了形态变化,细胞生长受到抑制,且呈时间和浓度依赖性,细胞周期被阻滞在S期。结论:EEP对食管癌EC-1.71细胞具有增殖抑制作用。Objective: To investigate the inhibitory effects of ethanol extracts of Physalis alkekengi Linn.(EEP) on the proliferation of human esophageal cancer EC-1.71 cell strain and to explore the possible mechanism.Methods: Human esophageal neoplasms EC-1.71 cells were treated with EEP;the morphological changes were inspected with inverted microscope;the inhibitory effects of EEP on the proliferation of human EC-1.71 cells was investigated using cell counting;the effects of EEP on the cell cycle of EC-1.71 cells were analyzed by flow cytomertry.Results: EEP treatment can significantly inhibit the proliferation in a dose-and time-dependent manner.Morphological changes of EC-1.71 cells were found after EEP treatment.EEP treatment arrested EC-1.71 cells at the S phase.Conclusion: EEP could inhibit the proliferation of EC-1.71 cells.

关 键 词：食管癌 酸浆醇提物 细胞周期 

分 类 号：R[医药卫生]