高氧性急性肺损伤大鼠模型的建立与评价  

Establishment and Evaluation of Rat Model of Hyperoxia Induced Acute Lung Injury

作　　者：石敏[1] 金国萍[1] 刘庆辉[1] 刘长庭[1] 俞森洋[1] 

机构地区：[1]解放军总医院南楼呼吸科,北京100853

出　　处：《中国呼吸与危重监护杂志》2011年第2期144-147,共4页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：北京市自然科学基金项目(编号:7082088)

摘　　要：目的建立高氧性急性肺损伤大鼠模型并进行评价。方法 80只大鼠随机分为空气组和高氧组,各组又随机分为12、24、36、48及60 h五个亚组。检测高氧(浓度在95%以上)暴露过程中大鼠动脉血气变化;肺组织HE染色观察肺损伤情况;测定肺湿/干(W/D)比、肺系数、肺通透指数及支气管肺泡灌洗液(BALF)中乳酸脱氢酶(LDH)活性、WBC计数。比较不同高氧暴露时间下大鼠的肺损伤程度,确定复制高氧性急性肺损伤大鼠模型的最佳条件。结果组织病理学显示随着高氧暴露时间的延长,大鼠肺组织损伤逐渐加重,在高氧暴露48～60 h时,出现典型的急性肺损伤的病理学特征。肺W/D比、肺系数、肺通透指数和BALF中LDH活性、WBC计数均进行性升高,于48 h升至5个观察时相的最高峰,60 h上述指标虽然与48 h比较无显著性差异,但数值上已开始有下降的趋势;PaO2呈进行性下降。结论高氧(浓度在95%以上)暴露48～60 h能够成功复制典型的高氧性急性肺损伤大鼠模型。Ojective To establish a rat model of hyperoxia induced acute lung injury.Methods Eighty healthy male SD rats were randomly divided into an air group and a hyperoxia group(≥95% O2).Each group was further divided into 12 h,24 h,36 h,48 h,60 h subgroups.Arterial blood gas was monitored.Lung tissue was sampled for evaluation of lung wet to dry ratio,lung index,and pulmonary permeation index.Bronchoalveolar lavage fluid(BALF) was collected for measurement of lactate dehydrogenase(LDH) activity and white blood cell count(WBC).Results After hyperoxia exposure for 48～60 h,lung pathology showed alveolar structure disruption,lung parenchyma wrath bleeding and edema.Lung wet to dry ratio,lung index,pulmonary permeation index,LDH and WBC in BALF all increased significantly,peaked at 48 h and remained at high level at 60 h while PaO2 dropped progressively.Conclusion Exposure to ≥95% O2 for 48～60 h can successfully establish the rat model of hyperoxia induced acute lung injury.

关 键 词：急性肺损伤 高氧 动物模型 大鼠 

分 类 号：R-332[医药卫生]