油菜蜂花粉中两种植物甾醇的制备及HPLC-ELSD法测定  

Preparation of Two Phytosterols from Rape Bee Pollen and Determination by HPLC-ELSD

作　　者：韦玮[1] 杨义芳[1] 

机构地区：[1]中国医药工业研究总院上海医药工业研究院,上海200040

出　　处：《中国医药工业杂志》2011年第6期416-418,422,共4页Chinese Journal of Pharmaceuticals

基　　金：国家"重大新药创制"科技重大专项(2009ZX09301-007);2009年上海市中药现代化专项资助项目(09dZ1975200);2008年上海市中药现代化专项资助项目(08DZ1971801);上海市自然科学基金资助项目(06ZR14078)

摘　　要：油菜蜂花粉水洗预处理后经索氏提取得到植物甾醇含量较高的脂肪油部位,通过皂化、乙醚萃取、重结晶和液相制备色谱获得两种植物甾醇,经波谱鉴定为24-亚甲基胆甾醇(1)和异岩藻甾醇(2)。其中2为首次从油菜蜂花粉中分离得到。以1、2为对照品,采用HPLC-ELSD法测定了不同油菜蜂花粉中两种植物甾醇的含量。The fatty oil with high content of phytosterols was obtained by Soxhlet extraction from rape bee pollen.The part of phytosterols was obtained after saponification and ether extraction.Two phytosterols,identified as 24-methylenecholesterol and isofucosterol by spectrum analysis,were separated by preparative liquid chromatography and recrystallization.Isofucosterol was separated from rape bee pollen for the first time.As control substances,the contents of these two phytosterols in different rape bee pollen were determined by HPLC-ELSD.

关 键 词：油菜蜂花粉 24-亚甲基胆甾醇 异岩藻甾醇 高效液相色谱-蒸发光检测器 测定 

分 类 号：R9[医药卫生—药学]