两株丝状真菌N3、N9的分类鉴定  

Identification of Strains N3 and N9 of Filamentous Fungi

作　　者：牛莉娜[1] 韩建荣[2] 王英[1] 汪洋[1] 杨婧[1] 张优 吴春华 

机构地区：[1]海南医学院热带医学与检验医学院,海南海口571101 [2]山西大学生命科学学院,山西太原030006

出　　处：《山西大学学报（自然科学版）》2011年第4期651-654,共4页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(31070048)

摘　　要：从一酸奶样品中分离到两株丝状真菌N3、N9,通过形态学观察、ITS序列测定及其系统发育分析,初步确定了N3、N9菌株的分类地位以及与其近缘种之间的生物系统学关系.实验结果表明N3、N9菌株在PDA培养基上形成典型绒状菌落,菌丝体上具有担子菌所特有的锁状联合结构.ITS序列测定及系统发育分析表明N3菌株与灰白侧耳(Pleurotus spodoleucus)同源性最高,N9菌株与佛罗里达侧耳(Pleurotus floridanus)同源性最高.The strains N3 and N9 of filamentous fungi were isolated from one yoghurt sample.By morphological observation,nucleotide sequence analysis of their internal transcribed spacer regions and 5.8SrDNA,the taxonomy position of strains N3,N9 and biosystematics of them and their close relative species were identified.The results showed that the strains N3,N9 on potato dextrose agar medium created circular and smooth fungal colonies.Their hyphae had distinct clamp connections structure,which was unique to basidiomycetes.The sequence analysis showed that the strain N3 and P.spodoleucus had the most close relationship,which suggested that they were probably conspecific.The strain N9 and P.floridanus were closely related,which indicated that N9 might belong to P.floridanus.

关 键 词：丝状真菌 ITS序列测定 灰白侧耳 佛罗里达侧耳 

分 类 号：N55[自然科学总论]