CpGODN增强人肺癌细胞株A549放射增敏作用的研究  

The mechanism of CpG ODN enhancing the radiosensitivity of lung cancer cell line A549

作　　者：颜伟[1] 孙梯业[2] 杨春敏[1] 贾敏[1] 李静[1] 史蕊[2] 唐和兰[1] 杜斌[1] 韩全利[1] 

机构地区：[1]空军总医院干部病房,100142 [2]第二炮兵总医院肝胆胃肠病研究所

出　　处：《中华临床医师杂志（电子版）》2011年第13期3768-3772,共5页Chinese Journal of Clinicians(Electronic Edition)

摘　　要：目的初步探讨CpG ODN增强人肺腺上皮细胞株A549放射增敏作用。方法 ELISA法检测细胞TNF-α、IL-12和INF-γ的分泌水平,Griess检测细胞NO的含量并观察CpG ODN1826与β射线诱导A549细胞AP-1活化的抑制作用。结果 CpGODN增加了人肺癌细胞株A549TNF-α、IL-12、INF-γ和NO的分泌,在联合β射线照射后对A549细胞的杀伤作用更加显著,并显著抑制A549细胞AP-1的活化。结论 CpG ODN对A549有明显的放射增敏作用,其机制可能与CpG ODN增强A549细胞分泌TNF-α、IL-12、INF-γ、NO和抑制A549细胞AP-1的活化有关。Objective To investigate the effect of CpG ODN on the radiosensitivity of lung epithelial cell line A549.Methods The TNF-α,IL-12 and INF-γ secretion by A549 were detected by ELISA;NO level was tested by Griess method,AP-1 activation within A549 cells was observed using electrophoretic mobility shift assay.Results The inhibitory role was enhanced when CpG ODN1826(10 μg/ml)were combined with β-ray irradiation,with the increase of TNF-α,IL-12 and INF-γ secretion by cells.CpG ODN1826 combined with β-ray irradiation increased NO level in A549 cells and inhibited the AP-1 activation within A549 cells.Conclusions CpG ODN1826 can increase the radiosensitivity of lung epithelial cell line A549 and may be tightly related to increasing secretions of IL-12,IFN-γ,TNF-α and NO from cells and the inhibition of AP-1 activation.

关 键 词：辐射耐受性 CpG ODN1826 A549 

分 类 号：R734.2[医药卫生—肿瘤]