五聚素3基因甲基化在食管鳞癌中的作用  

Effect of PTX3 gene methylation in human esophageal squamous cell carcinoma

作　　者：王俊雄[1] 杨硕[2] 贺远龙[3] 朱圣韬[4] 张澍田[4] 

机构地区：[1]首都医科大学附属北京友谊医院医疗保健中心,100050 [2]北京大学第三医院检验科 [3]青岛市立医院消化科 [4]首都医科大学附属北京友谊医院消化内科,100050

出　　处：《中华临床医师杂志（电子版）》2011年第16期4694-4698,共5页Chinese Journal of Clinicians(Electronic Edition)

摘　　要：目的研究食管鳞癌组织中五聚素3(PTX3)基因的表达,以及基因启动子区甲基化状态对其表达的影响。方法应用半定量逆转录PCR(RT-PCR)、甲基化特异性PCR(MSP)、免疫组织化学的方法对癌旁食管黏膜组织和食管鳞癌组织进行检测,分析PTX3基因mRNA的表达、基因启动子区甲基化状态和PTX3蛋白阳性表达与食管癌临床病理特征的关系。结果 25%(5/20)食管鳞癌组织和90%(18/20)正常食管黏膜组织存在PTX3基因表达,PTX3mRNA在食管癌与癌旁组织之间的表达差异有统计学意义(χ2=17.289,P<0.001)。80%(16/20)食管鳞癌组织和25%(5/20)正常食管黏膜组织存在PTX3基因CpG岛超甲基化,提示PTX3基因超甲基化与食管癌发生之间有关联(χ2=12.13,P<0.001)。PTX3蛋白在食管癌组织中表达的阳性率为30.38%(24/79),癌旁食管组织PTX3蛋白阳性表达率为84.81%(67/79)(P<0.001)。随着食管癌临床分期增加,PTX3蛋白阳性表达率逐渐降低。结论 PTX3基因启动子高甲基化是PTX3表达失活的主要原因,在食管鳞癌的发生发展中起重要作用,PTX3基因启动子甲基化可作为食管鳞癌预后评估的潜在肿瘤标志物。Objective The PTX3 gene was identified in our laboratory from esophageal squamous cell carcinoma(ESCC).Methods We examined PTX3 expression in 79 primary ESCCs and the corresponding non-malignant tissues,We further analyzed the correlation of PTX3 expression with the clinicopathological characteristics of ESCC patients.Results The low expression level was significantly associated with increased tumor stage in ESCC(P<0.001).Using methylation-specific PCR(MSP) and semi-quantitative reverse-transcription PCR,we found that PTX3 expression was downregulated due to the gene promoter hypermethylation.Methylation in tumor tissues(85%) was more frequent than adjacent non-malignant tissues(25%)(P<0.001).Conclusions The results reveal PTX3 as a novel candidate tumor suppressor gene downregulated through promoter hypermethylation in ESCC.

关 键 词：食管肿瘤 基因 抑制 DNA甲基化 五聚素3 

分 类 号：R735.1[医药卫生—肿瘤]