不同骨髓细胞成分经门静脉移植治疗大鼠肝纤维化的对照研究  

Transplantation of bone marrow cells through portal veins reduced liver fibrosis in mice

作　　者：陆勤平[1] 曹葆强[2] 魏伟[2] 

机构地区：[1]南京医科大学附属南京第一人民医院急诊科,210006 [2]安徽医科大学临床药物研究所博士后流动站

出　　处：《中华临床医师杂志（电子版）》2011年第17期4992-4996,共5页Chinese Journal of Clinicians(Electronic Edition)

基　　金：安徽省科技厅自然科学基金(090413255);安徽省人社厅博士后研究人员科研活动经费资助部(省)重点项目(皖人社秘[2010]460号)

摘　　要：目的以不同的骨髓细胞(BMC)成分经门静脉移植,对比其治疗大鼠肝纤维化的效果,为临床应用提供依据。方法采用四氯化碳(CCl4)综合法制作Wistar大鼠肝纤维化模型,8周后经病理检查证实成模者共60只进入实验。随机分为A、B、C三组,每组20只,A、B、C组大鼠分别经门静脉注入骨髓有核细胞、骨髓间质干细胞(MSC)和等体积的生理盐水。以上所有大鼠均在原环境下继续饲养4周后处死,切取肝组织分别作HE及Masson染色肝纤维化分级、羟脯氨酸含量测定、胶原纤维含量测定。结果三组肝脏羟脯氨酸含量分别为(0.5046±0.1288)g、(0.5710±0.4458)g、(0.5960±0.3418)g,A组与B组比较、A组与C组比较差异均有统计学意义(P<0.05),而B组与C组比较差异无统计学意义;图像分析大鼠肝脏纤维含量分别为(3.752±0.983)%、(4.842±1.225)%、(5.021±0.442)%,A组与B组比较、A组与C组比较差异均有统计学意义(P<0.05),而B组与C组比较差异无统计学意义。结论在CCl4诱导的大鼠肝纤维化模型中,骨髓有核细胞能缓解大鼠肝纤维化程度。Objective To study the effect of the bone marrow cells(BMC)transplanted through the portal vein in the hepatic cirrhosis rat models.Methods 60 hepatic cirrhosis rat models by carbon tetrachloride(CCl4)were divided into A,B,C three groups.500 μl(3×106)karyocytes from the bone marrow,500 μl(3×106)marrow stem cells(MSC)and 500 μl saline were respectively injected into the rats in group A,B,C through the portal veins.The rats in the groups of A,B,C were continually hypodermically injected CCl4 twice every week for 4 weeks.The behaviors and the reactions of each rat were recorded.After 4 weeks,all the rats were executed,and the mortality,the hydroxyproline(Hyp) and the pathological changes of the livers were compared.Results With the continual injection of CCl4,Hyp in A,B,C was 0.5046±0.1288,0.571±0.4458,0.596±0.3418 respectively.There was difference between group A and B,A and C(P<0.05)in Hyp;while no difference between group B and C(P>0.05).Conclusions Transplantation of the karyocytes from the bone marrow cells through portal vein could reduce liver fibrosis in mice on persistent damage.

关 键 词：骨髓移植 肝硬化 大鼠 Wistar 

分 类 号：R575.2[医药卫生—消化系统]