低氧对肝肾组织内红细胞生成素基因表达的影响  

Effects of acute hypoxia and intermittent hypoxic acclimatization on erythropoietin gene expression in rat hepatic and renal tissues

作　　者：陈伟[1] 陈家佩[1] 葛世丽[1] 从玉文[1] 付小兵 

机构地区：[1]军事医学科学院放射医学研究所,北京100850

出　　处：《感染．炎症．修复》2004年第3期86-88,共3页Infection Inflammation Repair

基　　金：国家自然科学基金重点课题(编号39730190);国家重点基础研究规划(编号 G1999054204)

摘　　要：目的:观察急性低氧和间断低氧习服对大鼠肝、肾组织内红细胞生成素(EPO)及其调控蛋白低氧诱导因子-1α(HIF-1α)基因表达的影响。方法:用低压舱模拟高原低氧环境,进行3km 2周,5km 两周低氧训练,使大鼠达到低氧习服状态后,采用 Northern 斑点杂交研究间断低氧习服前后,8km 4h 低氧处理的大鼠肝、肾组织内 EPO、HIF-1α基因表达的变化。结果:与常氧对照组相比,急性低氧处理的大鼠肝、肾组织内 EPO 基因表达都明显增强(P<0.05～0.01);而间断低氧习服后,同样的低氧处理的大鼠肝、肾组织中 EPO 基因表达都明显低于急性低氧组(P<0.01),mRNA 含量与常氧对照组水平差异不显著(P>0.05)。急性低氧处理的大鼠肝组织中,HIF-1α基因表达水平明显高于常氧对照组和间断低氧习服组(P<0.01),而在不同处理的肾组织中,该基因表达水平差异不明显(P>0.05)。结论:急性低氧能够刺激肝肾组织内 EPO 基因表达,而间断低氧习服的大鼠耐低氧能力明显增强,肝、肾组织内 EPO 基因表达水平恢复到常氧对照组水平。HIF-1α在低氧影响 EPO 基因表达的过程中可能起着重要的调节作用。To eplore the effects of acute hypoxia and intermittent hypoxic acclimatization on gene ex- pression of erythropoietin(EPO)and hypoxia-inducible factor-1α(HIF-1α)in rat hepatic and renal tissues.Meth- ods:Twenty-four rats(weight 180～220g)were divided into three groups:normal control group(NC),intermit- tent hypoxic acclimatization(IH)and acute hypoxia group(AH).Gene expression of EPO and HIF-1α were ex- amined with northern dot blot method.Results:As compared with NC group,the levels of EPO gene expression in rat hepatic and renal tissues in AH were both significantly elevated(P<0.05～0.01).In IH group,the contents of EPO mRNA in hepatic and renal tissues were apparently decreased versus AH group(P<0.01),whilst were not differential elevated in comparison with NC(P>0.05).In hepatic tissue from AH group,the content of HIF- 1α mRNA was more than those in NC group and IH group,while the levels of gene expression of HIF-1α were no substantial change in renal tissues from different groups(P>0.05).Conclusion:Hypoxic acclimatization can in- hibit the increment of EPO gene expression induced by acute hypoxia in rat hepatic and renal tissues,in which HIF-1α may play important roles.

关 键 词：急性低氧 间断低氧习服 红细胞生成素 低氧诱导因子-1Α 

分 类 号：R36[医药卫生—病理学]