急性低氧和低氧“习服”对人肝肿瘤细胞内血管内皮细胞生长因子和低氧诱导因子基因表达和蛋白含量的影响  

Effect of acute hypoxia and hypoxic"acclimatization"on VEGF and HIF-1α gene expression and protein content in HepG2 cells

作　　者：陈伟[1] 陈家佩[1] 葛世丽[1] 从玉文[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850

出　　处：《感染．炎症．修复》2002年第2期93-95,共3页Infection Inflammation Repair

基　　金：国家自然科学基金重点课题(39730190)

摘　　要：目的:观察急性低氧和低氧"习服"时,人肝肿瘤(HepG2)细胞内血管内皮生长因子(VEGF)和低氧诱导因子-1a(HIF-1a)基因表达和蛋白含量的变化。方法:细胞到达低氧"习服"状态后,用 Northern 杂交和Western 杂交检测细胞内 VEGF 和 HIF-1α基因表达以及蛋白含量的变化。结果:急性低氧能够诱导 VEGF、HIF-1α基因表达和胞内蛋白含量升高;而低氧"习服"后再连续低氧48h,细胞内 VEGF 与 HIF-1α的 mRNA 和蛋白含量都恢复到与对照相近的水平。结论:HepG2细胞达到低氧"习服"状态后,耐低氧的能力明显增强,减轻或抑制急性低氧对 VEGF 基因表达的促进作用,其中 HIF-1α可能起着重要的调节作用。Objective;To observe the influence of acute hypoxia and hypoxic'acclimatization'on VEGF and HIF-1a gene expression in HepG2 cells.Methods;HepG2 was cultured in 1% O_2 for 24 hours,then in21% O_2 for another 24 hours,composing a hypoxic training cycle.After 6 cycles,HepG2 cells came to the status of hypoxic 'acclimatization'.During the course of hypoxic training,gene transcription and translation of VEGF and HIF- 1α were estimated with Northern blot and Western blot.Results:Acute hypoxia could induce gene transcription and translation of VEGF and HIF-1α.After hypoxic'acclimatization',gene expression of VEGF and HIF-1a was near normal,while protein content of VEGF HIF-1a fell to control olxlue in the circumstance of 1% O_2 for 48h.Conclusion;When the status of hypoxic'acclimatization'was reached,the hypoxic endurance of HepG2 cells was significantly increased,the hypoxia-induced increment of VEGF gene transcription and translation in HepG2 cells were inhibited in which HIF-1α might play an important role.

关 键 词：急性低氧 低氧“习服” 血管内皮细胞生长因子 低氧诱导因子-1Α 

分 类 号：R735[医药卫生—肿瘤]