人血管内皮细胞生长因子融合表达质粒的构建  

Construction of a fusional expression plasmid of human vascular endothelial growth factor

作　　者：陈伟[1] 付小兵[1] 孙丹[1] 孙同柱[1] 孙小庆[1] 顾小曼[1] 盛志勇[1] 

机构地区：[1]解放军第304医院烧伤研究所基础部,北京100037

出　　处：《感染．炎症．修复》2000年第2期97-99,共3页Infection Inflammation Repair

基　　金：国家重大基础项目规划资助项目(CG1999054204);国家杰出青年科学基金(39525024)

摘　　要：目的:利用真核细胞表达载体pcDNA3.1(+)构建人血管内皮细胞生长因子融合表达质粒pcD-NA3-VEGF。方法:采用PCR技术和DNA重组技术。结果:将编码人VEGF基因的cDNA序列克隆至表达载体pcDNA3.1(+)上,DNA测序实验结果证明cDNA序列正确,cDNA序列是由Kozak序列,翻译起始密码ATG,信号序列,编码序列和终止密码子等部分组成。结论:通过研究获得了VEGF细胞因子的融合表达质粒pcDNA3-VEGF,为进一步研究VEGF表达载体进行转基因治疗奠定基础。Objective: To construct the plasmid of pcDNA3-VEGF for fusional expression of human vascular endothelial growth factor with eukaryotic expression vector pcDNA3.1(+). Methods: PCR and DNA recombination methods in vitro were used. Results:A gene fragment encoding VEGF was cloned into the fusional expression vector pcDNA3.1(+). DNA sequence analysis indicated that it was correct. The sequence of VEGF cDNA was composed of Kozak sequence, translation original codon, ATG, signal sequence,coding sequence and terminating codon. Conclusion: A fusional expression plasmid pcDNA3 VEGF was successful constructed. From the basis of the work, further study of transgenic therapy with VEGF could be carried out.

关 键 词：VEGF真核表达载体 克隆 

分 类 号：R[医药卫生]