乙型肝炎病毒基因S区准种特点与临床转归关系的初步研究  

preliminary study on association of hepatitis b virus s gene quasispecies with clinical outcome of hbv infection

作　　者：郁嘉伦[1] 于德敏[1] 姜节洪[1] 张东华[1] 黄素园[1] 刘峰[1] 张欣欣[1] 

机构地区：[1]上海瑞金医院感染科,200025

出　　处：《中华临床感染病杂志》2011年第1期-,共5页Chinese Journal of Clinical Infectious Diseases

摘　　要：目的 初步探讨hbv基因s区的准种特点与乙型肝炎病毒(hbv)感染临床转归的关系.方法 选择慢性hbv携带者、慢性乙型肝炎患者、慢性重型肝炎患者各3例,所有患者均为男性,且hbv基因型均为c型.扩增患者血清中hbv基因s区片段并克隆,每份样本挑选20个克隆进行测序,并用spss 15.0软件进行统计分析.结果 慢性hbv携带者以及慢性乙型肝炎患者的hbv基因s区准种复杂度小于慢性重型乙型肝炎患者,但差异无统计学意义(t=1.7,p=0.26).对于hbv s区t细胞表位的第45、47和85位氨基酸,慢性乙型肝炎患者的准种构成情况比hbv携带者复杂(p=0.01),与慢性重型乙型肝炎的差别无统计学意义(p=0.06),计算机模拟分析提示优势克隆和非优势克隆的t细胞表位均能与ctl受体有效结合.结论 hbv基因s区部分t细胞表位的准种构成差异可能与hbv慢性感染的临床转归相关. abstract： objective to investigate the association of hepatitis b virus(hbv) s gene quasispecies with the outcome of hbv infection.methods serum samples were collected from three chronic hbv carriers, three chronic hepatitis b and three chronic severe hepatitis b patients.all subjects were male and with hbv genotype c.hbv s gene was amplified, and 20 clones of hbv fragment were randomly selected and sequenced from each sample.spss 15.0 software was adopted for analysis.results quasispecies complexity of hbv s gene in chronic hbv carriers and chronic hepatitis b tended lower than that of the severe chronic hepatitis b, but the difference was not of statistical significance (p＞0.05).in t cell epitope 45, 47, 85 amino acid sites of the hbv s gene, the constitution of quasispecies in the chronic hepatitis b was more complex than that of the hbv carriers (p=0.01), but compared with the severe chronic hepatitis, the difference was not significant (p=0.06).the computer model showed that both the dominant clones and the non dominant clones could effectively bind to the receptors of cyObjective To investigate the association of hepatitis B virus(HBV) S gene quasispecies with the outcome of HBV infection.Methods Serum samples were collected from three chronic HBV carriers, three chronic hepatitis B and three chronic severe hepatitis B patients.All subjects were male and with HBV genotype C.HBV S gene was amplified, and 20 clones of HBV fragment were randomly selected and sequenced from each sample.SPSS 15.0 software was adopted for analysis.Results Quasispecies complexity of HBV S gene in chronic HBV carriers and chronic hepatitis B tended lower than that of the severe chronic hepatitis B, but the difference was not of statistical significance (P＞0.05).In T cell epitope 45, 47, 85 amino acid sites of the HBV S gene, the constitution of quasispecies in the chronic hepatitis B was more complex than that of the HBV carriers (P=0.01), but compared with the severe chronic hepatitis, the difference was not significant (P=0.06).The computer model showed that both the dominant clones and the non dominant clones could effectively bind to the receptors of cytotoxic T lymphocytes.Conclusion Quasispecies in some T cell epitopes of HBV S gene may be related with the clinical outcome of hepatitis B.

关 键 词：肝炎病毒 乙型 准种 突变 

分 类 号：R5[医药卫生—内科学]