微囊化肝细胞移植促进急性肝衰竭大鼠肝细胞再生的研究  

liver regeneration after transplantation of microencapsulated hepatecytes in rats with acute liver failure

作　　者：陈永平[1] 许烂漫[1] huang yu 王晓东[1] gao feng 

机构地区：[1]温州医学院附属第一医院感染科,325027 [2]department of infectious diseases, the first affiliated hospital of wenzhou medical college, wenzhou 325027, china

出　　处：《中华临床感染病杂志》2008年第2期-,共5页Chinese Journal of Clinical Infectious Diseases

摘　　要：目的 分析微囊化肝细胞移植对急性肝衰竭大鼠肝细胞再生的影响.方法 通过腹腔注射d-氨基半乳糖(d-gal)建立急性肝衰竭大鼠模型,18 h后将造模动物随机分成生理盐水组(Ⅰ)、裸肝细胞移植组(Ⅱ)和微囊化肝细胞移植组(Ⅲ).造模后6、12、24、36、48、72、120、168及240 h时,从各组随机抽取6只大鼠,留下腔静脉血观察肝功能变化,取肝组织用免疫组织化学法检测增殖细胞核抗原(pcna)的表达.另取6只正常大鼠的肝组织作为参考值.结果 Ⅰ、Ⅱ、Ⅲ组大鼠10 d生存率分别是26.7%(4/15)、40.0%(6/15)和73.3%(11/15),3组间大鼠生存率差异有统计学意义(x2=9.349,p=0.009).Ⅲ组大鼠生存率较Ⅰ、Ⅱ组有明显提高.造模后6 h,各组大鼠的alt、ast均有所升高,以36～72 h最为明显.Ⅱ、Ⅲ组的alt、ast自36 h开始下降,Ⅲ组的下降较Ⅱ组显著.Ⅰ组造模后tbil逐渐升高,72 h时达最高峰;Ⅱ、Ⅲ组的tbil在48 h时开始下降,其中,在36、48和72 h时Ⅲ组下降较Ⅱ组更为显著.免疫组织化学结果表明,正常组pcna蛋白呈弱阳性或阴性表达,造模后表达量逐渐增多,48 h时达最高峰.其中,Ⅲ组的表达量显著高于Ⅰ、Ⅱ组.结论 微囊化肝细胞移植促进了肝细胞的再生,并可改善急性肝衰竭大鼠的肝功能和预后. abstract： objective to investigate liver regeneration after transplantation of microencapsulated hepatocytes in rats with acute liver failure (alf). methods alf rat model was established by intraperitoneal injection of d-galactosamine (d-galn). after 18 h, rats were randomized into control group ( Ⅰ ), free hepatoeyte transplantation group ( Ⅱ ) and the microencapsulated hepatecyte transplantation group (Ⅲ). six rats for each group were randomly selected and sacrificed at 6, 12, 24, 36, 48, 72, 120, 168 and 240 h after alf induced and blood samples from inferior vena cava were collected. liver functions were tested in blood samples, and the expression of proliferating

关 键 词：增殖细胞核抗原 微囊化肝细胞 肝功能衰竭 急性 肝细胞再生 

分 类 号：R575[医药卫生—消化系统]