74例经拉米夫定治疗的乙型肝炎病毒感染者耐药情况分析  

p gene rt region mutations of hepatitis b virus in 74 patients with lamivudine-resistance

作　　者：张忠东[1] 刘惠敏[1] 吕铁锋[1] 

机构地区：[1]杭州市第六人民医院内二科,310014

出　　处：《中华临床感染病杂志》2008年第4期-,共3页Chinese Journal of Clinical Infectious Diseases

摘　　要：目的 探讨拉米夫定(lam)耐药hbv多聚酶基因区基因突变的分布及其与病毒载量之间的关系.方法 采用pcr方法对74例lam耐药患者血清hbv多聚酶基因区进行扩增,对pcr产物进行直接测序并检测病毒载量情况.通过描述性统计及多元统计分析hbv耐药基因突变情况及与病毒载量情况.结果 74例hbv感染者多聚酶基因区基因突变类型有7种,其中以rtm204v/rtl180m、rtm204i和rtm204i/rtl180m最为多见.hbv多聚酶基因区基因突变位点主要为rtm204i(38.7%)、rtm204v(21.8%)、rtl180m(38.7%)和rtv173l(0.8%).结论 lam耐药突变位点和类型复杂多样,常见hbv基因耐药位点及类型与病毒载量之间无直接相关性,但可能存在少数位点突变类型与病毒载量相关. abstract： objective to investigate mutations at hbv p gene rt region in lamivudine-resistant patients. methods serum samples were collected from 74 hepatitis b patients resistant to lamivudine. hbv p gene rt region was amplified by pcr, and the pcr products were directly sequenced and the viral loads were detected. results there were 7 forms of mutations in 74 patients harboring ymdd mutations detected in this study. the common mutation forms were rtm204v/rtl180m, rtm204i and rtm204i/rtl180m. the most frequent mutation positions were rtm204i (38.7%), rtm204v (21.8%), rtl180m (38.7%) and rtv173l (0.8%). conclusions the mutation forms and positions of lamivudine-resistant viral strains are complex. common mutation forms and positions are not directly correlated with the viral load, but some novel forms may be associated with it.

关 键 词：病毒 肝炎 乙型 拉米夫定 突变 病毒载量 

分 类 号：R9[医药卫生—药学]